Carbon from glucose-U-C was found to be incorporated into DNA, RNA and protein in HeLa cell cultures infected with poliovirus. This incorporation was partially inhibited by the 2nd hr of infection. The inhibition was related to the multiplicity of exposure to poliovirus and DNA was inhibited to a greater degree than RNA. In infected and normal HeLa cultures the rate of evolution of CO from glucose-1-C was 77 times greater than from glucose-6-C. The incorporation of C from these sugars into macromolecules in normal and infected cultures did not reflect this difference, thus the C in the macromolecules comes from the glycolytic pathway rather than the hexosemonophosphate shunt. Concentrations of guanidine inhibiting poliovirus replication also inhibit the incorporation of C from glucose-U-C into DNA and RNA in normal and infected cultures. In cultures receiving both virus and guanidine, the inhibitory effects of each were not additive, suggesting that the viral inhibition of C incorporation requires some early step in virus replication.


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