%0 Journal Article %A Graves, I. L. %T Incorporation of Radioactivity from 14C-Sugars into Macromolecules in Poliovirus-infected or Guanidine-treated HeLa Cells %D 1969 %J Journal of General Virology, %V 4 %N 1 %P 101-109 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-4-1-101 %I Microbiology Society, %X SUMMARY 14Carbon from glucose-U-14C was found to be incorporated into DNA, RNA and protein in HeLa cell cultures infected with poliovirus. This incorporation was partially inhibited by the 2nd hr of infection. The inhibition was related to the multiplicity of exposure to poliovirus and DNA was inhibited to a greater degree than RNA. In infected and normal HeLa cultures the rate of evolution of 14CO2 from glucose-1-14C was 77 times greater than from glucose-6-14C. The incorporation of 14C from these sugars into macromolecules in normal and infected cultures did not reflect this difference, thus the 14C in the macromolecules comes from the glycolytic pathway rather than the hexosemonophosphate shunt. Concentrations of guanidine inhibiting poliovirus replication also inhibit the incorporation of 14C from glucose-U-14C into DNA and RNA in normal and infected cultures. In cultures receiving both virus and guanidine, the inhibitory effects of each were not additive, suggesting that the viral inhibition of 14C incorporation requires some early step in virus replication. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-4-1-101