1887

Abstract

Summary

Cell lines of baby hamster kidney (BHK 21), green monkey kidney (Vero, MA 134), rhesus monkey kidney (MA 104), and rabbit kidney (MA hi) were found suitable for titration and multiplication of arboviruses. Plaque characteristics of 52 arboviruses and 20 virus strains and titres of some of them in BHK 21 and other cell lines were established, using one type of serum-free standard overlay and standard maintenance medium. However, the addition of cortisol or other compounds was necessary to prolong survival of BHK 21 cell sheets, and induce or improve plaque formation.

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1968-03-01
2022-01-26
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References

  1. Dulbecco R. 1952; Production of plaques in monolayer cultures by single particles of an animal virus. Proc. natn. Acad. Sei. U.S.A 38:747
    [Google Scholar]
  2. Dukes P. P., Sekeris C. E. 1965; Stimulierung des Einbaus von 2-l4C Uracil in Ribonucleinsaure von Rattenleberkemen durch Cortisol in vitro . Hoppe-Seylers Z. Physiol. Chem 341:149
    [Google Scholar]
  3. Feigelson M., Gross P. R., Feigelson P. 1961; Early effects of cortisone on nucleic acid and protein metabolism of rat liver. Biochim. biophys. Acta 55:495
    [Google Scholar]
  4. Fernándes M. V. 1959; Irradiation of cells in tissue culture. Z. Zellforsch. mikrosk. Anat 50:433
    [Google Scholar]
  5. Greenman D. L., Wicks W. D., Kenney F. T. 1965; Stimulation of ribonucleic acid synthesis by steroid hormones.II. J. biol. Chem 240:4420
    [Google Scholar]
  6. Hannoun C., Fernñndes M. V., Macieira-Coelho A. 1965; Effects of cortisone on in vitroviral infection.I. Proc. Soc. exp. Biol. Med 119:153
    [Google Scholar]
  7. Hayflick L., Moorhead P. S. 1961; The serial cultivation of human diploid cell strains. Expl Cell Res 25:585
    [Google Scholar]
  8. Hayflick L., Jacobs P., Perkins F. 1964; A procedure for the standardization of tissue culture media. Nature; Lond: 204146
    [Google Scholar]
  9. Hellman A., Kalter S. S. 1963; Hydrocortisone stress effect on attenuated and virulent Polio virus synthesis. Fedn Proc. Fedn Am. Socs exp. Biol 22:558
    [Google Scholar]
  10. Holden M., Adams L. B. 1962; The influence of hydrocortisone on vaccinia virus grown in L cells. J. infect. Dis 110:268
    [Google Scholar]
  11. Karabatsos N., Buckley S. M. 1967; Susceptibility of the baby-hamster kidney-cell line (BHK-21) to infection with arboviruses. Am. J. trop. Med. Hyg 16:99
    [Google Scholar]
  12. Kenney F. T., Kull F. J. 1963; Hydrocortisone-stimulated synthesis of nuclear RNA in enzyme induction. Proc. natn. Acad. Sci. U.S.A 50:493
    [Google Scholar]
  13. Kenney F. T., Wicks W. D., Greenman D. L. 1965; Hydrocortisone stimulation of RNA synthesis in induction of hepatic enzymes. J. cell. comp. Physiol 66:125
    [Google Scholar]
  14. Kilbourne E. D. 1952; Paradoxical effects of cortisone on influenza B virus infection of chick embryo. J. clin. Invest 31:643
    [Google Scholar]
  15. Kilbourne E. D., Horsfall F. L. 1951; Increased virus in eggs injected with cortisone. Proc. Soc. exp. Biol. Med 76:116
    [Google Scholar]
  16. Kilbourne E. D., Smart K. M., Pokorny B. A. 1961; Inhibition by cortisone of the synthesis and action of interferon. Nature; Lond: 190650
    [Google Scholar]
  17. Macieira-Coelho A., Fernández M. V. 1963; Action of cortisone on human embryonic diploid cell strains. Fedrt Proc. Fedn Am. Socs exp. Biol 22:382
    [Google Scholar]
  18. Macieira-Coelho A., Fernandez M. V., Hellman W. J. 1965; Cortisone on in vitro viral infection. Proc. Soc. exp. Biol. Med 119:631
    [Google Scholar]
  19. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones-an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  20. De Maeyer E., De Maeyer J. 1963; Two-sided effect of steroids on interferon in tissue culture. Nature; Lond: 197724
    [Google Scholar]
  21. Mendelson J., Finland M. 1966; Effects of cortisol on interferon production by mouse peritoneal leukocytes and spleen in vitro and in vivo . Proc. Soc. exp. Biol. Med 123:98
    [Google Scholar]
  22. Miles J. A. R., Austin F. J. 1963; Growth of arboviruses in BHK 21 cells. Aust. J.Sci 25:466
    [Google Scholar]
  23. Sigel M. M., Beasley E. R. 1955; Demonstration of two effects of cortisone on virus-cell interaction. Proc. Soc. exp. Biol. Med 88:86
    [Google Scholar]
  24. Stewart R. B. 1960; Effect of cortisone on the growth of psittacosis virus in cultures of L cells. J. Bact. 80:25
    [Google Scholar]
  25. Wicks W. D., Greenman D. L., Kenney F. T. 1965; Stimulation of ribonucleic acid synthesis by steroid hormones. J. biol. Chem 240:4414
    [Google Scholar]
  26. Yasumura Y., Kawakita Y. 1963; Research into SV 40 by tissue culture. Nippon Rinsho 21:1201
    [Google Scholar]
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