1887

Abstract

A bacteriophage (IF3) capable of mediating generalized transduction in strain Db11 has been isolated and characterized. The genome of this strain has recently been sequenced and is likely to become the reference strain for researchers. IF3 is most likely a virulent phage, which can transduce markers at frequencies of 10 transductants per p.f.u. It has a lipopolysaccharide receptor and was determined to have a latent period of 50 min and a burst size of approximately 100 phages. The phage DNA was resistant to digestion with restriction enzymes. Electron microscopy showed IF3 to be a member of the family . This is the first report of a generalized transducing phage able to infect Db11 and this phage will be a valuable tool for functional genomic analysis of the pathogen host.

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2006-06-01
2019-10-15
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References

  1. Ackermann, H. W. ( 2003; ). Bacteriophage observations and evolution. Res Microbiol 154, 245–251.[CrossRef]
    [Google Scholar]
  2. Aucken, H. M. & Pitt, T. L. ( 1998; ). Antibiotic resistance and putative virulence factors of Serratia marcescens with respect to O and K serotypes. J Med Microbiol 47, 1105–1113.[CrossRef]
    [Google Scholar]
  3. Flyg, C., Kenne, K. & Boman, H. G. ( 1980; ). Insect pathogenic properties of Serratia marcescens: phage-resistant mutants with a decreased resistance to Cecropia immunity and a decreased virulence to Drosophila. J Gen Microbiol 120, 173–181.
    [Google Scholar]
  4. Grimont, P. A. & Grimont, F. ( 1978; ). The genus Serratia. Annu Rev Microbiol 32, 221–248.[CrossRef]
    [Google Scholar]
  5. Hava, D. L. & Camilli, A. ( 2001; ). Isolation and characterization of a temperature-sensitive generalized transducing bacteriophage for Vibrio cholerae. J Microbiol Methods 46, 217–225.[CrossRef]
    [Google Scholar]
  6. Hayes, W. ( 1970; ). The Genetics of Bacteria and their Viruses, 2nd edn. Oxford: Blackwell Scientific Publications.
  7. Hejazi, A. & Falkiner, F. R. ( 1997; ). Serratia marcescens. J Med Microbiol 46, 903–912.[CrossRef]
    [Google Scholar]
  8. Kurz, C. L., Chauvet, S., Andres, E. & 13 other authors ( 2003; ). Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening. EMBO J 22, 1451–1460.[CrossRef]
    [Google Scholar]
  9. Lazzaro, B. P., Sceurman, B. K. & Clark, A. G. ( 2004; ). Genetic basis of natural variation in D. melanogaster antibacterial immunity. Science 303, 1873–1876.[CrossRef]
    [Google Scholar]
  10. Mallo, G. V., Kurz, C. L., Couillault, C., Pujol, N., Granjeaud, S., Kohara, Y. & Ewbank, J. J. ( 2002; ). Inducible antibacterial defense system in C. elegans. Curr Biol 12, 1209–1214.[CrossRef]
    [Google Scholar]
  11. Masters, M. ( 1985; ). Generalized transduction. In Genetics of Bacteria, pp. 197–215. Edited by J. Scaife, D. Leach & A. Galizzi. London: Academic Press.
  12. Palomar, J., Guasch, J. F., Regue, M. & Vinas, M. ( 1990; ). The effect of nuclease on transformation efficiency in Serratia marcescens. FEMS Microbiol Lett 57, 255–258.
    [Google Scholar]
  13. Palomar, J., Montilla, R., Fuste, M. C. & Vinas, M. ( 1993; ). The role of O-antigen in susceptibility of Serratia marcescens to non-immune serum. Microbios 76, 189–196.
    [Google Scholar]
  14. Regue, M., Fabregat, C. & Vinas, M. ( 1991; ). A generalized transducing bacteriophage for Serratia marcescens. Res Microbiol 142, 23–27.[CrossRef]
    [Google Scholar]
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T. A. ( 1989; ). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Sapriel, G., Wandersman, C. & Delepelaire, P. ( 2003; ). The SecB chaperone is bifunctional in Serratia marcescens: SecB is involved in the Sec pathway and required for HasA secretion by the ABC transporter. J Bacteriol 185, 80–88.[CrossRef]
    [Google Scholar]
  17. Schulenburg, H. & Ewbank, J. J. ( 2004; ). Diversity and specificity in the interaction between Caenorhabditis elegans and the pathogen Serratia marcescens. BMC Evol Biol 4, 49.[CrossRef]
    [Google Scholar]
  18. Swift, S., Winson, M. K., Chan, P. F. & 11 other authors ( 1993; ). A novel strategy for the isolation of luxI homologues: evidence for the widespread distribution of a LuxR : LuxI superfamily in enteric bacteria. Mol Microbiol 10, 511–520.[CrossRef]
    [Google Scholar]
  19. Yang, Y. J., Wu, P. J. & Livermore, D. M. ( 1990; ). Biochemical characterization of a beta-lactamase that hydrolyzes penems and carbapenems from two Serratia marcescens isolates. Antimicrob Agents Chemother 34, 755–758.[CrossRef]
    [Google Scholar]
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