1887

Abstract

Although mycoplasmas possess a very limited genome, little is known about their virulence mechanisms and methods of persistence in the host. Examination of a wide range of mycoplasma species found considerable variation in their ability to form a biofilm. , , , and produced prolific biofilms. Conversely, the highly pathogenic mycoplasma and causative agent of contagious bovine pleuropneumonia, subsp. SC, was unable to produce a biofilm. Biofilms were found to be considerably more resistant to stress, including heat and desiccation, than planktonic cells. A link between the biofilm phenotype and genotype as determined by molecular typing was found for . Analysis of biofilms using fluorescent staining combined with confocal microscopy demonstrated that mycoplasma biofilms formed a highly differentiated structure with stacks and channels. Biofilm formation may indicate that mycoplasmas are capable of surviving in the environment.

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2006-04-01
2024-12-14
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References

  1. Almeida R. A, Rosenbusch R. F. 1991; Capsule like surface material of Mycoplasma dispar induced by in vitro growth in culture with bovine cells is antigenically related to similar structures expressed in vivo. Infect Immun 59:3119–3125
    [Google Scholar]
  2. Brennan P. C, Feinstein R. N. 1969; Relationship of hydrogen peroxide production by Mycoplasma pulmonis to virulence for catalase-deficient mice. J Bacteriol 98:1036–1040
    [Google Scholar]
  3. Brenner C, Wroblewski H, Le Henaff M, Montagnier L, Blanchard A. 1997; Spiralin, a mycoplasmal membrane lipoprotein, induces T-cell-independent B-cell blastogenesis and secretion of proinflammatory cytokines. Infect Immun 65:4322–4329
    [Google Scholar]
  4. Costerton J, Stewart W. P. S, Greenberg E. P. 1999; Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322 [CrossRef]
    [Google Scholar]
  5. Donlan R. M, Costerton J. W. 2002; Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 15:167–193 [CrossRef]
    [Google Scholar]
  6. Dunne W. M Jr. 2002; Bacterial adhesion: seen any good biofilms lately?. Clin Microbiol Rev 15:155–166 [CrossRef]
    [Google Scholar]
  7. Fonseca A. P, Extremina C, Fonseca A. F, Sousa J. C. 2004; Effect of subinhibitory concentration of piperacillin/tazobactam on Pseudomonas aeruginosa . J Med Microbiol 53:903–910 [CrossRef]
    [Google Scholar]
  8. Herbelin A, Ruuth E, Delorme D, Michel-Herbelin C, Praz F. 1994; Mycoplasma arginini TUH-14 membrane lipoproteins induce production of interleukin-1, interleukin-6, and tumor necrosis factor alpha by human monocytes. Infect Immun 62:4690–4694
    [Google Scholar]
  9. Joseph L. A, Wright A. C. 2004; Expression of Vibrio vulnificus capsular polysaccharide inhibits biofilm formation. J Bacteriol 186:889–893 [CrossRef]
    [Google Scholar]
  10. Mah T. F, O'Toole G. A. 2001; Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol 9:34–39 [CrossRef]
    [Google Scholar]
  11. March J. B, Kerr K, Lema B. 2003; Rapid detection of contagious bovine pleuropneumonia by a Mycoplasma mycoides subsp . mycoides SC capsular polysaccharide-specific antigen detection latex agglutination test. Clin Diagn Lab Immunol 10:233–240
    [Google Scholar]
  12. McAuliffe L, Kokotovic B, Ayling R. D, Nicholas R. A. 2004; Molecular epidemiological analysis of Mycoplasma bovis isolates from the United Kingdom shows two genetically distinct clusters. J Clin Microbiol 42:4556–4565 [CrossRef]
    [Google Scholar]
  13. Miles A. A, Misra S. S. 1938; The estimation of the bactericidal power of the blood. J Hyg 36:732–748
    [Google Scholar]
  14. Miles R. J, Taylor R. R, Varsani H. 1991; Oxygen uptake and H[sub]2[/sub]O[sub]2[/sub] production by fermentative Mycoplasma spp. Med Microbiol 34:219–223 [CrossRef]
    [Google Scholar]
  15. Minga U. M. 1981; Smooth and rough colony types of Mycoplasma mycoides subsp. mycoides : in vitro characterisation and pathogenicity in cattle. Tanzania Vet Bull 3:53–62
    [Google Scholar]
  16. Niang M, Rosenbusch R. F, Andrews J. J, Kaeberle M. L. 1998; Demonstration of a capsule on Mycoplasma ovipneumoniae . Am J Vet Res 59:557–562
    [Google Scholar]
  17. Nicholas R, Baker S. 1998; Recovery of mycoplasmas from animals. Methods Mol Biol 104:37–43
    [Google Scholar]
  18. O'Toole G. A, Kolter R. 1998; Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol 30:295–304 [CrossRef]
    [Google Scholar]
  19. Otto K, Silhavy T. J. 2002; Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway. Proc Natl Acad Sci U S A 99:2287–2292 [CrossRef]
    [Google Scholar]
  20. Rachid S, Ohlsen K, Witte W, Hacker J, Ziebuhr W. 2000; Effect of subinhibitory antibiotic concentrations on polysaccharide intercellular adhesin expression in biofilm-forming Staphylococcus epidermidis . Antimicrob Agents Chemother 44:3357–3363 [CrossRef]
    [Google Scholar]
  21. Razin S, Yogev D, Naot Y. 1998; Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 62:1094–1156
    [Google Scholar]
  22. Sachse K, Grajetzki C, Rosengarten R, Hanel I, Heller M, Pfutzner H. 1996; Mechanisms and factors involved in Mycoplasma bovis adhesion to host cells. Zentralbl Bakteriol 284:80–92 [CrossRef]
    [Google Scholar]
  23. Sachse K, Helbig J. H, Lysnyansky I, Grajetzki C, Muller W, Jacobs E, Yogev D. 2000; Epitope mapping of immunogenic and adhesive structures in repetitive domains of Mycoplasma bovis variable surface lipoproteins. Infect Immun 68:680–687 [CrossRef]
    [Google Scholar]
  24. Sasaki T, Shintani M, Kihara K. 1984; Inhibition of growth of mammalian cell cultures by extracts of arginine-utilizing mycoplasmas. In Vitro 20:369–375 [CrossRef]
    [Google Scholar]
  25. Stewart P. S. 2002; Mechanisms of antibiotic resistance in bacterial biofilms. Int J Med Microbiol 292:107–113 [CrossRef]
    [Google Scholar]
  26. Tajima M, Yagihashi T, Miki Y. 1982; Capsular material of Mycoplasma gallisepticum and its possible relevance to the pathogenic process. Infect Immun 36:830–833
    [Google Scholar]
  27. Thomas A, Sachse K, Farnir F, Dizier I, Mainil J, Linden A. 2003; Adherence of Mycoplasma bovis to bovine bronchial epithelial cells. Microb Pathog 34:141–148 [CrossRef]
    [Google Scholar]
  28. Tu A. H, Clapper B, Schoeb T. R, Elgavish A, Zhang J, Liu L, Yu H, Dybvig K. 2005; Association of a major protein antigen of Mycoplasma arthritidis with virulence. Infect Immun 73:245–249 [CrossRef]
    [Google Scholar]
  29. Valdivieso-Garcia A, Rosendal S, Serebrin S. 1989; Adherence of Mycoplasma mycoides subspecies mycoides to cultured endothelial cells. Zentralbl Bakteriol 272:210–215 [CrossRef]
    [Google Scholar]
  30. Woese C. R, Maniloff J, Zablen L. B. 1980; Phylogenetic analysis of the mycoplasmas. Proc Natl Acad Sci U S A 77:494–498 [CrossRef]
    [Google Scholar]
  31. Woese C. R, Olsen G. J, Ibba M, Soll D. 2000; Aminoacyl-tRNA synthetases, the genetic code, and the evolutionary process. Microbiol Mol Biol Rev 64:202–236 [CrossRef]
    [Google Scholar]
  32. Yi K, Rasmussen A. W, Gudlavalleti S. K, Stephens D. S, Stojiljkovic I. 2004; Biofilm formation by Neisseria meningitidis . Infect Immun 72:6132–6138 [CrossRef]
    [Google Scholar]
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