1887

Abstract

A novel shuttle entrapment vector, pMMB2, was used to identify a large transposable element, Tn (44·3 kb), of DSM 11072. Tn has a composite structure with divergently oriented copies of a cryptic transposon, Tn (Tn family), located at both termini. The core region of the element contains a large set of putative genes, whose products show similarity to enzymes involved in central intermediary metabolism (e.g. tricarboxylic acid cycle or 2-methylcitrate cycle), transporters, transcriptional regulators and conserved proteins of unknown function. A 4·2 kb DNA segment of Tn is homologous to a region of chromosome cII of 2.4.1, which exemplifies the mosaic structure of this element. Tn is bordered by 5 bp long directly repeated sequences and is located within a mega-sized replicon, pWKS5, in the DSM 11072 genome. Spontaneous inversion of the core region of Tn was detected in the host genome. Analysis of the distribution of Tn in three other strains of revealed that this element was present exclusively within DSM 11072, which suggests its relatively recent acquisition by lateral transfer. The identification of Tn (which may be considered a transposable genomic island) provides evidence for the transposition and lateral transfer of large DNA segments of chromosomal origin (carrying various housekeeping genes), which may have a great impact on the evolution of bacterial genomes.

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2006-04-01
2020-04-08
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