1887

Abstract

utilizes several xenosiderophores under conditions of iron limitation, including the citrate hydroxamate siderophore aerobactin. Analysis of the genome sequence revealed the presence of two genes, (PA4675) and PA1365, encoding proteins displaying significant similarity to the aerobactin outer-membrane receptor, IutA, of . The and PA1365 genes were mutated by insertional inactivation and it was demonstrated that ChtA is the outer-membrane receptor for aerobactin. ChtA also mediated the utilization of rhizobactin 1021 and schizokinen, which are structurally similar to aerobactin. In contrast to the utilization of other xenosiderophores by , there was no apparent redundancy in the utilization of aerobactin, rhizobactin 1021 and schizokinen. The utilization of citrate hydroxamate siderophores by was demonstrated to be TonB1 dependent. A Fur box was identified in the region directly upstream of and it was demonstrated by the Fur titration assay that this region is capable of binding Fur and accordingly that expression of is iron regulated. The PA1365 mutant was unaffected in the utilization of citrate hydroxamate siderophores.

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2006-04-01
2019-11-19
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