1887

Abstract

The predicted chromosomal origin of replication () from the alfalfa symbiont is shown to allow autonomous replication of a normally non-replicating plasmid within cells. This is the first chromosomal replication origin to be experimentally localized in the and its location, adjacent to , is the same as for in , the only experimentally characterized alphaproteobacterial . Using an electrophoretic mobility shift assay and purified DnaA replication initiation protein, binding sites for DnaA were mapped in the region. Mutations in these sites eliminated autonomous replication. that expressed DnaA from a plasmid promoter was observed to form pleomorphic filamentous cells, suggesting that cell division was perturbed. Interestingly, this cell phenotype is reminiscent of differentiated bacteroids found inside plant cells in alfalfa root nodules.

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2006-02-01
2020-10-27
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