1887

Abstract

is a food-borne pathogen associated increasingly with human gastroenteritis. has a high prevalence in swine, but is isolated also from cattle and poultry. Multilocus sequence typing (MLST) systems have been developed to differentiate strains. Although substantial allelic diversity was identified across all seven MLST loci, no correlations were made in two previous studies between allele or sequence type (ST) and the source of the organism. However, this may be due to either the relatively small number or the low diversity of strains used to validate both MLST studies. This study describes the typing of 488 strains from 4 different food animal sources (cattle, chickens, swine and turkeys), collected at different times over a 6 year period from different USA geographical locations. A total of 149 STs were identified. The 185 swine strains were the most diverse, possessing 82 STs. The cattle strains were the most clonal; 52/63 (83 %) strains possessed a single ST (ST-1068). A subpopulation of strains, collected primarily from turkeys, was identified, containing both - and -associated MLST alleles, specifically the allele . The majority of STs and alleles were host associated, i.e. found primarily in strains from a single food-animal source. Only 12/149 (8 %) STs were found in multiple sources. Additionally, the majority (34/46, 74 %) of major (>5) alleles were more prevalent in certain hosts (swine, poultry). The presence of host-associated MLST alleles could lead potentially to more efficient source tracking in this species, especially in the trace-back of both sporadic and outbreak human clinical strains to animal sources.

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2006-01-01
2019-10-15
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vol. , part 1, pp. 245 - 255

Strain information, allelic profiles, and sequence types of C. coli animal strains. [ PDF file] (1545 KB)



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