1887

Abstract

It is well established that motility is an essential virulence trait of the human gastric pathogen . Accordingly, chemotaxis contributes to the ability of to colonize animal infection models. Chemotactic signal transduction in differs from the enterobacterial paradigm in several respects. In addition to a separate CheY response regulator protein (CheY1), contains a CheY-like receiver domain (CheY2) which is C-terminally fused to the histidine kinase CheA. Furthermore, the genome of encodes three CheV proteins consisting of an N-terminal CheW-like domain and a C-terminal receiver domain, while there are no orthologues of the chemotaxis genes , and . To obtain insight into the mechanisms controlling the chemotactic response of , we investigated the phosphotransfer reactions between the purified two-component signalling modules . We demonstrate that both CheY1 and CheY2 are phosphorylated by CheA∼P and that the three CheV proteins mediate the dephosphorylation of CheA∼P, but with a clearly reduced efficiency as compared to CheY1 and CheY2. Furthermore, our data indicate retrophosphorylation of CheAY2 by CheY1∼P, suggesting a role of CheY2 as a phosphate sink to modulate the half-life of CheY1∼P.

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2005-10-01
2019-10-15
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