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Volume 151, Issue 8

Influence of fusaric acid on phenazine-1-carboxamide synthesis and gene expression of strain PCL1391 Free

Abstract

Production of the antifungal metabolite phenazine-1-carboxamide (PCN) by strain PCL1391 is essential for the suppression of tomato foot and root rot caused by the soil-borne fungus f. sp. . The authors have shown previously that fusaric acid (FA), a phytotoxin produced by , represses the production of PCN and of the quorum-sensing signal -hexanoyl--homoserine lactone (C-HSL). Here they report that PCN repression by FA is maintained even during PCN-stimulating environmental conditions such as additional phenylalanine, additional ferric iron and a low Mg concentration. Constitutive expression of or increases the production of C-HSL and abolishes the repression of PCN production by FA. Transcriptome analysis using PCL1391 microarrays showed that FA represses expression of the phenazine biosynthetic operon () and of the quorum-sensing regulatory genes and . FA does not alter expression of the PCN regulators , and . In conclusion, reduction of PCN levels by FA is due to direct or indirect repression of and . Microarray analyses identified genes of which the expression is strongly influenced by FA. Genes highly upregulated by FA are also upregulated by iron starvation in . This remarkable overlap in the expression profile suggests an overlapping stress response to FA and iron starvation.

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  • Published Online:
Keyword(s): C6-HSL, N-hexanoyl-l-homoserine lactone , FA, fusaric acid , HSL, homoserine lactone and PCN, phenazine-1-carboxamide
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2005-08-01
2024-05-01
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Influence of fusaric acid on phenazine-1-carboxamide synthesis and gene expression of Pseudomonas chlororaphis strain PCL1391
Microbiology 151, 2805 (2005); https://doi.org/10.1099/mic.0.28063-0
/content/journal/micro/10.1099/mic.0.28063-0
/content/journal/micro/10.1099/mic.0.28063-0
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