1887

Abstract

Acid resistance (AR) in is important for its survival in the human gastrointestinal tract and involves three systems. The first AR system is dependent on the sigma factor RpoS. The second system (the GAD system) requires the glutamate decarboxylase isoforms encoded by the and genes. The third system (the ARG system) requires the arginine decarboxylase encoded by . Loss of topoisomerase I function from deletion or Tn insertion mutations lowered the resistance to killing by pH 2 or 2·5 treatment by 10-fold to >100-fold. The RpoS and GAD systems were both affected by the mutation, but the ARG system of AR was not affected. Northern blot analysis showed that induction of and transcription in stationary phase and at pH 5·5 was decreased in the mutant. Western blot analysis showed that the mutation did not affect accumulation of RpoS, GadX or GadW proteins. Topoisomerase I might have a direct influence on the transcription of AR genes. This influence does not involve R-loop formation as the overexpression of RNase H did not alleviate the decrease of AR caused by the mutation. The effect of the mutation could be suppressed by an mutation, so topoisomerase I might be required to counteract the effect of H-NS protein on gene expression, in addition to its influence on RpoS-dependent transcription.

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2005-08-01
2020-04-01
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