1887

Abstract

Humoral immune responses of active TB patients against six mycobacterial lipid antigens [trehalose 6,6′-dimycolate (TDM) from BCG (TDM-T) and complex (TDM-M), trehalose 6-monomycolate (TMM) from BCG (TMM-T) and complex (TMM-M), triacyl (PL-2) and tetraacyl (PL-1) phosphatidylinositol dimannosides] were examined by ELISA. IgG antibodies of TB patients with active disease reacted against the six lipid antigens distinctively, but heterogeneously. If tests were combined and an overall positive was scored cumulatively when any one of the six tests was positive, a good discrimination between patient and normal subject was obtained. A positive result in any one of the six tests was obtained in 91·5 % of all 924 hospitalized patients and 93·3 % of 210 patients at their first visit to the outpatient clinic. The IgG antibody response differed considerably from patient to patient, and the response patterns were grouped into several types. IgG antibody levels paralleled the bacterial burden; however, the smear-negative (culture-positive) patient group also showed high positive rates and mean ELISA Δ values against the six lipid antigens. There were also marked differences in positive rate and mean Δ values between cavity-positive and -negative groups, the former being higher than the latter. After anti-TB chemotherapy was initiated, IgG antibody levels decreased dramatically, paralleling the decrease in the amount of excretion of bacteria. Since multiple-antigen ELISA using particular lipid antigens was highly sensitive, and IgG antibody levels vary greatly at different stages of the disease, this technique is applicable for early diagnosis of smear-negative (and -positive) active TB and the prognosis for completion of anti-TB chemotherapy.

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2005-06-01
2024-12-07
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References

  1. Arloing S. 1898; Agglutination de bacille de la tuberculose vraie. C R Acad Sci 126:1398–1400
    [Google Scholar]
  2. Beckman E. M., Porcelli S. A., Morita C. T., Behar S. M., Furlong S. T., Brenner M. B. 1994; Recognition of a lipid antigen by CD1-restricted alpha beta+ T cells. Nature 372:691–694 [CrossRef]
    [Google Scholar]
  3. Benjamin R. G., Daniel T. M. 1982; Serodiagnosis of tuberculosis using the enzyme-linked immunoabsorbent assay (ELISA) of antibody to Mycobacterium tuberculosis antigen 5. Am Rev Respir Dis 126:1013–1016
    [Google Scholar]
  4. Benjamin R. G., Debanne S. M., Ma Y., Daniel T. M. 1984; Evaluation of mycobacterial antigens in an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of tuberculosis. J Med Microbiol 18:309–318 [CrossRef]
    [Google Scholar]
  5. Chan S. L., Reggiardo Z., Daniel T. M., Girling D. J., Mitchison D. A. 1990; Serodiagnosis of tuberculosis using an ELISA with antigen 5 and a hemagglutination assay with glycolipid antigens. Results in patients with newly diagnosed pulmonary tuberculosis ranging in extent of disease from minimal to extensive. Am Rev Respir Dis 142:385–389 [CrossRef]
    [Google Scholar]
  6. Chan E. D., Heifets L., Iseman M. D. 2000; Immunologic diagnosis of tuberculosis. Tuber Lung Dis 80:131–140 [CrossRef]
    [Google Scholar]
  7. Daniel T. M., Debanne S. M. 1987; The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme-linked immunosorbent assay. Am Rev Respir Dis 135:1137–1151
    [Google Scholar]
  8. Enomoto K., Oka S., Fujiwara N., Okamoto T., Okuda Y., Maekura R., Kuroki T., Yano I. 1998; Rapid serodiagnosis of Mycobacterium avium-intracellulare complex infection by ELISA with cord factor (trehalose 6,6′-dimycolate), and serotyping using the glycopeptidolipid antigen. Microbiol Immunol 42:689–696 [CrossRef]
    [Google Scholar]
  9. Fujiwara N., Oka S., Ide M., Kashima K., Honda T., Yano I. 1999; Production and partial characterization of antibody to cord factor (trehalose 6,6′-dimycolate) in mice. Microbiol Immunol 43:785–793 [CrossRef]
    [Google Scholar]
  10. Grange J. M., Gibson J., Nassau E., Kardjito T. 1980; Enzyme-linked immunosorbent assay (ELISA): a study of antibodies to Mycobacterium tuberculosis in the IgG, IgA and IgM classes in tuberculosis, sarcoidosis and Crohn's disease. Tubercle 61:145–152 [CrossRef]
    [Google Scholar]
  11. Harrington J. J. 3rd, Ho J. L., Lapa e Silva J. R., Conde M. B., Kritski A. L., Fonseca L. S., Saad M. H. 2000; Mycobacterium tuberculosis lipid antigens: use of multi-antigen based enzyme immunoassay for free and complex dissociated antibodies. Int J Tuberc Lung Dis 4:161–167
    [Google Scholar]
  12. He H., Oka S., Han Y. K., Yamamura Y., Kusunose E., Kusunose M., Yano I. 1991; Rapid serodiagnosis of human mycobacteriosis by ELISA using cord factor (trehalose-6,6′-dimycolate) purified from Mycobacterium tuberculosis as antigen. FEMS Microbiol Immunol 3:201–204
    [Google Scholar]
  13. Iinuma Y., Senda K., Takakura S. & 8 other authors; 2002; Evaluation of a commercially available serologic assay for antibodies against tuberculosis-associated glycolipid antigen. Clin Chem Lab Med 40:832–836
    [Google Scholar]
  14. Julian E., Cama M., Martinez P., Luquin M. 2001; An ELISA for five glycolipids from the cell wall of Mycobacterium tuberculosis: Tween 20 interference in the assay. J Immunol Methods 251:21–30 [CrossRef]
    [Google Scholar]
  15. Julian E., Matas L., Alcaide J., Luquin M. 2004; Comparison of antibody responses to a potential combination of specific glycolipids and proteins for test sensitivity improvement in tuberculosis serodiagnosis. Clin Diagn Lab Immunol 11:70–76
    [Google Scholar]
  16. Kardjito T., Handoyo I., Grange J. M. 1982; Diagnosis of active tuberculosis by immunological methods. 1. The effect of tuberculin reactivity and previous BCG vaccination on the antibody levels determined by ELISA. Tubercle 63:269–274 [CrossRef]
    [Google Scholar]
  17. Kashima K., Oka S., Tabata A., Yasuda K., Kitano A., Kobayashi K., Yano I. 1995; Detection of anti-cord factor antibodies in intestinal tuberculosis for its differential diagnosis from Crohn's disease and ulcerative colitis. Dig Dis Sci 40:2630–2634 [CrossRef]
    [Google Scholar]
  18. Kawamura M., Sueshige N., Imayoshi K., Yano I., Maekura R., Kohno H. 1997; Enzyme immunoassay to detect antituberculous glycolipid antigen (anti-TBGL antigen) antibodies in serum for diagnosis of tuberculosis. J Clin Lab Anal 11:140–145 [CrossRef]
    [Google Scholar]
  19. Kiran U., Shriniwas, Kumar R., Sharma A. 1985; Efficacy of three mycobacterial antigens in the serodiagnosis of tuberculosis. Eur J Respir Dis 66:187–195
    [Google Scholar]
  20. Kitada S., Maekura R., Toyoshima N., Fujiwara N., Yano I., Ogura T., Ito M., Kobayashi K. 2002; Serodiagnosis of pulmonary disease due to Mycobacterium avium complex with an enzyme immunoassay that uses a mixture of glycopeptidolipid antigens. Clin Infect Dis 35:1328–1335 [CrossRef]
    [Google Scholar]
  21. Kitada S., Maekura R., Toyoshima N., Naka T., Fujiwara N., Kobayashi M., Yano I., Ito M., Kobayashi K. 2005; Use of glycopeptidolipid core antigen for serodiagnosis of Mycobacterium avium complex pulmonary disease in immunocompetent patients. Clin Diagn Lab Immunol 12:44–51
    [Google Scholar]
  22. Lyashchenko K., Manca C., Colangeli R., Heijbel A., Williams A., Gennaro M. L. 1998; Use of Mycobacterium tuberculosis complex-specific antigen cocktails for a skin test specific for tuberculosis. Infect Immun 66:3606–3610
    [Google Scholar]
  23. Lyashchenko K. P., Singh M., Colangeli R., Gennaro M. L. 2000; A multi-antigen print immunoassay for the development of serological diagnosis of infectious diseases. J Immunol Methods 242:91–100 [CrossRef]
    [Google Scholar]
  24. Maekura R., Nakagawa M., Nakamura Y. 9 other authors 1993; Clinical evaluation of rapid serodiagnosis of pulmonary tuberculosis by ELISA with cord factor (trehalose-6,6′-dimycolate) as antigen purified from Mycobacterium tuberculosis . Am Rev Respir Dis 148:997–1001 [CrossRef]
    [Google Scholar]
  25. Maekura R., Okuda Y., Nakagawa M. 10 other authors 2001; Clinical evaluation of anti-tuberculous glycolipid immunoglobulin G antibody assay for rapid serodiagnosis of pulmonary tuberculosis. J Clin Microbiol 39:3603–3608 [CrossRef]
    [Google Scholar]
  26. Maekura R., Kohno H., Hirotani A., Okuda Y., Ito M., Ogura T., Yano I. 2003; Prospective clinical evaluation of the serologic tuberculous glycolipid test in combination with the nucleic acid amplification test. J Clin Microbiol 41:1322–1325 [CrossRef]
    [Google Scholar]
  27. Moody D. B., Reinhold B. B., Guy M. R. 9 other authors 1997; Structural requirements for glycolipid antigen recognition by CD1b-restricted T cells. Science 278:283–286 [CrossRef]
    [Google Scholar]
  28. Pan J., Fujiwara N., Oka S., Maekura R., Ogura T., Yano I. 1999; Anti-cord factor (trehalose 6,6′-dimycolate) IgG antibody in tuberculosis patients recognizes mycolic acid subclasses. Microbiol Immunol 43:863–869 [CrossRef]
    [Google Scholar]
  29. Porcelli S., Morita C. T., Brenner M. B. 1992; CD1b restricts the response of human CD4-8-T lymphocytes to a microbial antigen. Nature 360:593–597 [CrossRef]
    [Google Scholar]
  30. Reggiardo Z., Vazquez E., Schnaper L. 1980; ELISA tests for antibodies against mycobacterial glycolipids. J Immunol Methods 34:55–60 [CrossRef]
    [Google Scholar]
  31. Sada E., Brennan P. J., Herrera T., Torres M. 1990; Evaluation of lipoarabinomannan for the serological diagnosis of tuberculosis. J Clin Microbiol 28:2587–2590
    [Google Scholar]
  32. Sakai J., Matsuzawa S., Usui M., Yano I. 2001; New diagnostic approach for ocular tuberculosis by ELISA using the cord factor as antigen. Br J Ophthalmol 85:130–133 [CrossRef]
    [Google Scholar]
  33. Silva V. M., Kanaujia G., Gennaro M. L., Menzies D. 2003; Factors associated with humoral response to ESAT-6, 38 kDa and 14 kDa in patients with a spectrum of tuberculosis. Int J Tuberc Lung Dis 7:478–484
    [Google Scholar]
  34. Simonney N., Bourrillon A., Lagrange P. H. 2000; Analysis of circulating immune complexes (CICs) in childhood tuberculosis: levels of specific antibodies to glycolipid antigens and relationship with serum antibodies. Int J Tuberc Lung Dis 4:152–160
    [Google Scholar]
  35. Ulrichs T., Moody D. B., Grant E., Kaufmann S. H., Porcelli S. A. 2003; T-cell responses to CD1-presented lipid antigens in humans with Mycobacterium tuberculosis infection. Infect Immun 71:3076–3087 [CrossRef]
    [Google Scholar]
  36. WHO 2001; Modelling the impact of new diagnostic technologies in disease endemic countries. TBDI Activity Summary May 20011–5
    [Google Scholar]
  37. Zeiss C. R., Radin R. C., Williams J. E., Levitz D., Phair J. P. 1982; Detection of immunoglobulin G antibody to purified protein derivative in patients with tuberculosis by radioimmunoassay and enzyme-linked immunosorbent assay. J Clin Microbiol 15:93–96
    [Google Scholar]
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