1887

Abstract

( ) is an essential gene implicated in cell division and chromosome segregation. This gene was disrupted by insertional inactivation creating JHSR1, which was viable only when a wild-type copy of was expressed , confirming the essentiality of the gene. The absence of DivIVA in JHSR1 inhibited proper cell division, which resulted in abnormal cell clusters possessing enlarged cells of altered shape instead of the characteristic diplococcal morphology of enterococci. The lower viability of the mutant is caused by improper nucleoid segregation and impaired septation within the numerous cells generated in each cluster. Overexpression of DivIVA in KJB24 resulted in enlarged cells with disrupted cell division, suggesting that this round mutant strain could be used as an indicator for functionality of DivIVA. A mutant was not complemented by DivIVA, indicating that this protein does not recognize DivIVA-specific target sites in , or that it does not interact with other proteins of the cell division machinery of this micro-organism. DivIVA also failed to complement a mutant, supporting the phylogenetic distance between and . Our results indicate that DivIVA is a species-specific multifunctional protein implicated in cell division and chromosome segregation in .

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2005-05-01
2019-10-22
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