1887

Microbiology

Volume 151, Issue 4

The expression profile K-12 in response to minimal, optimal and excess copper concentrations Free

Abstract

The gene expression profile of K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 μM copper-glycine, the expression of both the and copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response.

  • Received:
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  • Published Online:
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2005-04-01
2019-10-13

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The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations
Microbiology 151, 1187 (2005); https://doi.org/10.1099/mic.0.27650-0
/content/journal/micro/10.1099/mic.0.27650-0
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