1887

Abstract

is a Gram-negative rod that inhabits the aquatic environment and is the aetiological agent of cholera, a disease that is endemic in much of Southern Asia. The 57·3 kb pathogenicity island-2 (VPI-2) is confined predominantly to toxigenic O1 and O139 serogroup isolates and encodes 52 ORFs (VC1758 to VC1809), which include homologues of an integrase (VC1758), a restriction modification system, a sialic acid metabolism gene cluster (VC1773–VC1783), a neuraminidase (VC1784) and a gene cluster that shows homology to Mu phage. In this study, a 14·1 kb region of VPI-2 comprising ORFs VC1773 to VC1787 was identified by PCR and Southern blot analyses in all 17 isolates examined. The VPI-2 region in was inserted adjacent to a serine tRNA similar to VPI-2 in . In 11 of the 17 isolates examined, an additional 5·3 kb region encoding VC1758 and VC1804 to VC1809 was present adjacent to VC1787. The evolutionary history of VPI-2 was reconstructed by comparative analysis of the (VC1784) gene tree with the species gene tree, deduced from the housekeeping gene malate dehydrogenase (), among and isolates. Both gene trees showed an overall congruence; on both gene trees O1 and O139 serogroup isolates clustered together, whereas non-O1/non-O139 serogroup isolates formed separate divergent branches with similar clustering of strains within the branches. One exception was noted: on the gene tree, sequences formed a distinct divergent lineage from sequences; however, on the gene tree, clustered with non-O1/non-O139 isolates, suggesting horizontal transfer of this region between these species.

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2005-01-01
2019-11-17
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