1887

Abstract

and () are adjacent regions located in DNA. encodes glucose kinase (Glk), which has been implicated in carbon catabolite repression (CCR) in the genus . In this work, the and genes from were used, either individually or together, to transform three mutants of var. resistant to CCR. These mutants present decreased levels of Glk, and deficiency in glucose transport. When the mutants were transformed with a plasmid containing the sequence, glucose uptake and Glk activity values were increased to levels similar to or higher than those of the original strain, and each strain regained sensitivity to CCR. This result was surprising considering that the putative amino acid sequence does not seem to encode a glucose permease or a Glk. In agreement with these results, an increase in mRNA levels was observed in a CCR-resistant mutant transformed with compared with those of the original strain and the CCR-resistant mutant itself. As expected, recombinants containing the sequence reverted Glk to normal activity values, but glucose uptake remained deficient. The data suggest that the gene product enhances transcription of both genes, and support the first specific role for this region in species. The physiological consequence of this effect is an increase in the glucose catabolites that may be involved in eliciting CCR in this genus.

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2005-05-01
2020-01-26
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