1887

Abstract

The gene of encodes a protein Fbl that is 58 % identical to the clumping factor A (ClfA) of . The gene was present in eight clinical isolates of . When Fbl was expressed on the surface of it promoted adherence to immobilized fibrinogen and cell clumping in a fibrinogen solution. Purified recombinant Fbl region A bound to immobilized fibrinogen in a dose-dependent manner and inhibited the adherence of both Fbl-expressing and ClfA-expressing strains of to fibrinogen. Adherence of and Fbl to immobilized fibrinogen was also inhibited by rabbit anti-Fbl region A antibodies and rabbit anti-ClfA region A antibodies, as well as by human immunoglobulin with a high level of anti-ClfA antibodies. Alignment of the A domains of CflA and Fbl revealed that all of the ClfA residues implicated in binding to the -chain of fibrinogen are conserved in Fbl. Nevertheless Fbl had a tenfold lower affinity for fibrinogen, suggesting that sequence differences that occur elsewhere in the protein, possibly in -strand E of domain N2, affect ligand binding.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.27337-0
2004-11-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/11/mic1503831.html?itemId=/content/journal/micro/10.1099/mic.0.27337-0&mimeType=html&fmt=ahah

References

  1. Bae T., Schneewind O. 2003; The YSIRK-G/S motif of staphylococcal protein A and its role in efficiency of signal peptide processing. J Bacteriol185:2910–2919[CrossRef]
    [Google Scholar]
  2. Bellamy R., Barkham T. 2002; Staphylococcus lugdunensis infection sites: predominance of abscesses in the pelvic girdle region. Clin Infect Dis35:E32–34[CrossRef]
    [Google Scholar]
  3. Davis S. L., Gurusiddappa S., McCrea K. W., Perkins S., Höök M. 2001; SdrG, a fibrinogen-binding bacterial adhesin of the microbial surface components recognizing adhesive matrix molecules subfamily from Staphylococcus epidermidis, targets the thrombin cleavage site in the Bβ chain. J Biol Chem276:27799–27805[CrossRef]
    [Google Scholar]
  4. Deivanayagam C. C., Wann E. R., Chen W., Carson M., Rajashankar K. R., Narayana S. V, Höök M.. 2002; A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A. EMBO J21:6660–6672[CrossRef]
    [Google Scholar]
  5. Donvito B., Etienne J., Denoroy L., Greenland T., Benito Y., Vandenesch F. 1997; Synergistic hemolytic activity of Staphylococcus lugdunensis is mediated by three peptides encoded by a non-agr genetic locus. Infect Immun65:95–100
    [Google Scholar]
  6. Dufour P., Jarraud S., Vandenesch F., Greenland T., Novick R. P., Bes M., Etienne J., Lina G. 2002; High genetic variability of the agr locus in Staphylococcus species. J Bacteriol184:1180–1186[CrossRef]
    [Google Scholar]
  7. Duthie E. S., Lorenz L. L. 1952; Staphylococcal coagulase; mode of action and antigenicity. J Gen Microbiol6:95–107[CrossRef]
    [Google Scholar]
  8. Freney J., Brun Y., Bes M., Meugnier H., Grimont F., Grimont P., Nervi C., Fleurette J. 1988; Staphylococcus lugdunensis sp. nov and Staphylococcus schleiferi sp. nov., two species from human clinical specimens. Int J Syst Bacteriol38:168–172[CrossRef]
    [Google Scholar]
  9. Hartford O., Francois P., Vaudaux P., Foster T. J. 1997; The dipeptide repeat region of the fibrinogen-binding protein (clumping factor) is required for functional expression of the fibrinogen-binding domain on the Staphylococcus aureus cell surface. Mol Microbiol25:1065–1076[CrossRef]
    [Google Scholar]
  10. Hartford O. M., Wann E. R., Foster T. J, Höök M.. 2001; Identification of residues in the Staphylococcus aureus fibrinogen-binding MSCRAMM clumping factor A (ClfA) that are important for ligand binding. J Biol Chem276:2466–2473[CrossRef]
    [Google Scholar]
  11. Josefsson E., Hartford O., O'Brien L., Patti J. M., Foster T. 2001; Protection against experimental Staphylococcus aureus arthritis by vaccination with clumping factor A, a novel virulence determinant. J Infect Dis184:1572–1580[CrossRef]
    [Google Scholar]
  12. Kragsbjerg P., Bomfim-Loogna J., Tornqvist E., Soderquist B. 2000; Development of antimicrobial resistance in Staphylococcus lugdunensis during treatment – report of a case of bacterial arthritis, vertebral osteomyelitis and infective endocarditis. Clin Microbiol Infect6:496–499[CrossRef]
    [Google Scholar]
  13. Leung M. J., Nuttall N., Pryce T. M., Coombs G. W., Pearman J. W. 1998; Colony variation in Staphylococcus lugdunensis. J Clin Microbiol36:3096–3098
    [Google Scholar]
  14. Mazmanian S. K., Ton-That H., Schneewind O. 2001; Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus. Mol Microbiol40:1049–1057[CrossRef]
    [Google Scholar]
  15. McDevitt D., Francois P., Vaudaux P., Foster T. J. 1994; Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus. Mol Microbiol11:237–248[CrossRef]
    [Google Scholar]
  16. McDevitt D., Francois P., Vaudaux P., Foster T. J. 1995; Identification of the ligand-binding domain of the surface-located fibrinogen receptor (clumping factor) of Staphylococcus aureus. Mol Microbiol16:895–907[CrossRef]
    [Google Scholar]
  17. McDevitt D., Nanavaty T., House-Pompeo K., Bell E., Turner N., McIntire L., Foster T., Höök M. 1997; Characterization of the interaction between the Staphylococcus aureus clumping factor (ClfA) and fibrinogen. Eur J Biochem247:416–424[CrossRef]
    [Google Scholar]
  18. Medved L., Litvinovich S., Ugarova T., Matsuka Y., Ingham K. 1997; Domain structure and functional activity of the recombinant human fibrinogen γ-module (γ148–411). Biochemistry36:4685–4693[CrossRef]
    [Google Scholar]
  19. Miyoshi A., Poquet I., Azevedo V., Commissaire J., Bermudez-Humaran L., Domakova E., Le Loir Y., Oliveira S. C., Gruss A., Langella P. 2002; Controlled production of stable heterologous proteins in Lactococcus lactis. Appl Environ Microbiol68:141–146
    [Google Scholar]
  20. Moreillon P., Entenza J. M., Francioli P., McDevitt D., Foster T. J., Francois P., Vaudaux P. 1995; Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis. Infect Immun63:4738–4743
    [Google Scholar]
  21. O'Brien L. M., Kerrigen S. W., Kaw G., Hogan M., Penades J., Litt D., Fitzgerald D. J., Foster T. J., Cox D. 2002; Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus aureus. Roles for the clumping factors ClfA and ClfB, the serine-aspartate repeat protein SdrE and protein A. Mol Microbiol44:1033–1044[CrossRef]
    [Google Scholar]
  22. Patel R., Piper K. E., Rouse M. S., Uhl J. R., Cockerill F. R., Steckelberg J. M. 2000; Frequency of isolation of Staphylococcus lugdunensis among staphylococcal isolates causing endocarditis: a 20-year experience. J Clin Microbiol38:4262–4263
    [Google Scholar]
  23. Perry A. M., Ton-That H., Mazmanian S. K., Schneewind O. 2002; Anchoring of surface proteins to the cell wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring. J Biol Chem277:16241–16248[CrossRef]
    [Google Scholar]
  24. Ponnuraj K., Bowden M. G., Davis S., Gurusiddappa S., Moore D., Choe D., Xu Y., Narayana S. V, Höök M.. 2003; A ‘dock, lock, and latch’ structural model for a staphylococcal adhesin binding to fibrinogen. Cell115:217–228[CrossRef]
    [Google Scholar]
  25. Poquet I., Saint V., Seznec E., Simoes N., Bolotin A., Gruss A. 2000; HtrA is the unique surface housekeeping protease in Lactococcus lactis and is required for natural protein processing. Mol Microbiol35:1042–1051[CrossRef]
    [Google Scholar]
  26. van der Mee-Marquet N., Achard A., Mereghetti L., Danton A., Minier M., Quentin R. 2003; Staphylococcus lugdunensis infections: high frequency of inguinal area carriage. J Clin Microbiol41:1404–1409[CrossRef]
    [Google Scholar]
  27. Vernachio J., Bayer A. S., Le T. & 7 other authors. 2003; Anti-clumping factor A immunoglobulin reduces the duration of methicillin-resistant Staphylococcus aureus bacteremia in an experimental model of infective endocarditis. Antimicrob Agents Chemother47:3400–3406[CrossRef]
    [Google Scholar]
  28. Wells J. M., Wilson P. W., Le Page R. W. F. 1993; Improved cloning vectors and transformation procedures for Lactococcus lactis. J Appl Bacteriol74:629–636[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.27337-0
Loading
/content/journal/micro/10.1099/mic.0.27337-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error