1887

Abstract

, an endophytic fungus originally isolated from , produces a mixture of volatile organic compounds (VOCs) in culture and its spectrum of antimicrobial activity is broad. Using the original isolate of as a selection tool, it has been possible to find other culturally and biochemically unique wild-type isolates of this organism existing as endophytes in a variety of other plant species, including (fern-leafed grevillea), (snake vine) and (nanka bakarra) growing in the northern reaches of the Northern Territory of Australia. Interestingly, none of the new isolates had a culture morphology that was identical to the original isolate, nevertheless each possessed hyphal characteristics that resembled that isolate. Furthermore, their ITS-5·8S rDNA sequences were 96–99 % identical to that of and the isolates were considered on the basis of the DNA sequence data. However, the VOCs produced by these new isolates greatly differed in quality from the original strain by virtue of the production of naphthalene, naphthalene, 1,1′-oxybis-, and one or more other compounds. In bioassays with a range of test micro-organisms, including fungi and bacteria, each isolate possessed biological activity but the range of activity was great. Artificial mixtures of some of the VOCs mimicked the effects of the VOCs of the fungus. The value of these observations to the biology and practical uses of in agriculture and other applications is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.27334-0
2004-12-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/12/mic1504023.html?itemId=/content/journal/micro/10.1099/mic.0.27334-0&mimeType=html&fmt=ahah

References

  1. Daisy, B., Strobel, G., Ezra, D., Castillo, U., Baird, G. & Hess, W. M.(2002a).Muscodor vitigenus sp. nov., an endophyte from Paullinia paullinoides. Mycotaxon 84, 39–50. [Google Scholar]
  2. Daisy, B. H., Strobel, G. A., Castillo, U., Ezra, D., Sears, J., Weaver, D. K. & Runyon, J. B.(2002b). Naphthalene, an insect repellent, is produced by Muscodor vitigenus, a novel endophytic fungus. Microbiology 148, 3737–3741. [Google Scholar]
  3. Ezra, D. & Strobel, G. A.(2003). Effect of substrate on the biobioactivity of volatile antimicrobials produced by Muscodor albus. Plant Sci 165, 1229–1238.[CrossRef] [Google Scholar]
  4. McAfee, B. J. & Taylor, A.(1999). A review of the volatile metabolites of fungi found on wood substrates. Nat Toxins 7, 283–303.[CrossRef] [Google Scholar]
  5. Mercier, J. & Jimenez, J. I.(2004). Control of decay of apples and peaches by the biofumigant fungus Muscodor albus. Postharvest Biol Technol 31, 1–8.[CrossRef] [Google Scholar]
  6. Sopalun, K., Strobel, G. A., Hess, W. M. & Worapong, J.(2003). A record of Muscodor albus, an endophyte from Myristica fragrans, in Thailand. Mycotaxon 88, 239–247. [Google Scholar]
  7. Stinson, M., Ezra, D., Hess, W. M., Sears, J. & Strobel, G. A.(2003a). An endophytic Gliocladium sp. of Eucryphia cordifolia producing selective volatile antimicrobial compounds. Plant Sci 165, 913–922.[CrossRef] [Google Scholar]
  8. Stinson, A. M., Zidack, N. K., Strobel, G. A. & Jacobsen, B. J.(2003b). Effect of mycofumigation with Muscodor albus and Muscodor roseus on seedling diseases of sugarbeet and Verticillium wilt of eggplant. Plant Dis 87, 1349–1354.[CrossRef] [Google Scholar]
  9. Strobel, G. A. & Daisy, B.(2003). Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67, 491–502.[CrossRef] [Google Scholar]
  10. Strobel, G. A., Dirkse, E., Sears, J. & Markworth, C.(2001). Volatile antimicrobials from Muscodor albus, a novel endophytic fungus. Microbiology 147, 2943–2950. [Google Scholar]
  11. Worapong, J., Strobel, G. A., Ford, E. J., Li, J. Y., Baird, G. & Hess, W. M.(2001).Muscodor albus anam. nov., an endophyte from Cinnamomum zeylanicum. Mycotaxon 79, 67–79. [Google Scholar]
  12. Worapong, J., Strobel, G. A., Daisy, B., Castillo, U., Baird, G. & Hess, W. M.(2002).Muscodor roseus anna. nov., an endophyte from Grevillea pteridifolia. Mycotaxon 81, 463–475. [Google Scholar]
/content/journal/micro/10.1099/mic.0.27334-0
Loading
/content/journal/micro/10.1099/mic.0.27334-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 4023 - 4031

Nucleotide sequence comparisons in the ITS-5.8S rDNA between the different isolates of are shown in the accompanying PDF.



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error