1887

Abstract

In the mycotoxigenic oilseed pathogens and and the model filamentous fungus , unsaturated fatty acids and their derivatives act as important developmental signals that affect asexual conidiospore, sexual ascospore and/or sclerotial development. To dissect the relationship between lipid metabolism and fungal development, an Δ-desaturase mutant that was unable to convert oleic acid to linoleic acid and was thus impaired in polyunsaturated fatty acid biosynthesis was generated. The Δ-desaturase mutant demonstrates delayed spore germination, a twofold reduction in growth, a reduced level of conidiation and complete loss of sclerotial development, compared to the wild-type. Host colonization is impaired, as reflected by a decrease in conidial production on live peanut and corn seed by the mutant compared to the wild-type. Similarly, the previously isolated Δ-desaturase mutant has reduced colonization capabilities compared to the wild-type. Therefore, desaturation mutants display a key requisite that affords a genetic solution to oilseed crop contamination by mycotoxigenic species: a reduction in the production of conidia, the infectious particle of the pathogenic aspergilli.

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2004-09-01
2019-11-14
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