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Volume 150, Issue 7

The gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by Free

Abstract

The ability of to acquire iron from the hostile environment of the host is known to be necessary for virulence and appears to be achieved using a similar system to that described for . In , high-affinity iron uptake is dependent upon the acquisition of copper. The authors have previously identified a gene () that encodes a copper transporter. Deletion of this gene results in a mutant strain that grows predominantly as pseudohyphae and displays aberrant morphology in low-copper conditions. This paper demonstrates that invasive growth by is induced by low-copper conditions and that this is augmented in a -null strain. It also shows that deletion of results in defective iron uptake. In , genes that facilitate high-affinity copper uptake are controlled by a copper-sensing transactivator, Mac1p. The authors have now identified a gene () that encodes a copper-sensing transactivator. A -null mutant displays phenotypes similar to those of a -null mutant and has no detectable transcripts in low-copper conditions. It is proposed that high-affinity copper uptake by is necessary for reductive iron uptake and is transcriptionally controlled by Mac1p in a similar manner to that in .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): BCS, bathocuproinedisulphonic acid , BPS, bathophenanthrolinedisulphonic acid , CuRE, copper-responsive element and PPD, p-phenylenediamine
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2004-07-01
2025-01-16
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/content/journal/micro/10.1099/mic.0.27004-0
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The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1
Microbiology 150, 2197 (2004); https://doi.org/10.1099/mic.0.27004-0
/content/journal/micro/10.1099/mic.0.27004-0
/content/journal/micro/10.1099/mic.0.27004-0
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