1887

Abstract

Nitrogen fixation is catalysed by some photosynthetic bacteria. This paper presents a phylogenetic comparison of a nitrogen fixation gene () with the aim of elucidating the processes underlying the evolutionary history of . In the NifH phylogeny, strains of were placed in close association with spp. and other phototrophic purple non-sulfur bacteria belonging to the -, separated from its close relatives and the phototrophic rhizobia ( spp. IRBG 2, IRBG 228, IRBG 230 and BTAi 1) as deduced from the 16S rRNA phylogeny. The close association of the strains of with those of and , as well as , was supported by the mol% G+C of their gene and by the signature sequences found in the sequence alignment. In contrast, comparison of a number of informational and operational genes common to CGA009, USDA 110 and 2.4.1 suggested that the genome of is more related to that of than to the genome. These results strongly suggest that the of is highly related to those of the phototrophic purple non-sulfur bacteria included in this study, and might have come from an ancestral gene common to these phototrophic species through lateral gene transfer. Although this finding complicates the use of to infer the phylogenetic relationships among the phototrophic bacteria in molecular diversity studies, it establishes a framework to resolve the origins and diversification of nitrogen fixation among the phototrophic bacteria in the -.

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2004-07-01
2019-10-22
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