1887

Abstract

A family of plasmid-borne DNA fragments of different length, apparently inherited from an ancient plasmid, has been identified in the world population of environmental strains. These fragments, named PPFs (arental lasmid DNA ragments), were ⩾99·8 % identical to each other in the common regions, and contained in their central region a variant of an aberrant mercury-resistance transposon (Tn ) that has lost its transposition genes. As a rule, recombinogenic elements were found at the breakpoints of identity between the different PPFs. Of these recombinogenic elements, a newly identified IS family element, a transposon, or a resolvase gene interrupted one end of the PPFs. At the opposite end, the breakpoint of some PPFs was mapped to the recombination point within, in each case, a different variant of a site (RS2), whilst in other PPFs, this end was eroded by insertion of a newly identified IS family element. On the basis of DNA sequence data, possible mechanisms of translocation of defective Tn -like elements via recombination events implicating the nearby (resolution) site and IS element are proposed.

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2004-04-01
2019-11-18
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vol. , part 4, pp. 979 - 992

Supplementary tableshowing genetic similarities for the determinants identified within the regions flanking the Hg loci.



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