1887

Abstract

It has recently been shown that the enteropathogen has an -linked general rotein ycosylation pathway (Pgl) that modifies many of the organism's proteins. To determine the role of the -linked general glycosylation in , the authors studied the gene, which shows high similarity to a family of sugar transferases. mutants were constructed in strains 81116 and 11168H. Both mutants were shown to be deficient in their ability to glycosylate a number of proteins, but their lipooligosaccharide and capsule were unaffected. The mutants had significantly reduced ability to adhere to and invade human epithelial Caco-2 cells. Additionally, the 81116 mutant was severely affected in its ability to colonize chicks. These results suggest that glycosylation is important for the attachment of to human and chicken host cells and imply a role for glycoproteins in the pathogenesis of .

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2004-06-01
2019-10-24
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