1887

Abstract

The entomopathogenic fungus has been successfully used in the control of several insect pests. Asexually produced spores (conidia) are the means for dispersal and transmission of the entomopathogen; upon contact with the insect cuticle they germinate and penetrate the host. In model fungal systems it has been found that phototropism, resetting of the circadian rhythm, the induction of carotenogenesis and the development of reproductive structures are controlled by blue light. The effect of light quality on conidial yield of was investigated. Incubation in total darkness resulted in continued vegetative growth and lack of reproductive structures. In contrast, growth of the fungus in continuous illumination or under a night–day regime resulted in prolific formation of conidiophores bearing abundant mature conidia. Conidiation was photoinduced in competent mycelia by a single pulse of blue light and colonies were competent only after they had grown at least 72 h under total darkness. The fluence–response curves generated with blue light indicated that the minimal fluence required for the photomorphogenetic response was 180 μmol m and the half-maximal response was at 400 μmol m. A fluence of 540 μmol m was enough to saturate the system, inducing the maximum production of 2·12×10 conidia per colony. Higher light intensities markedly decreased conidiation, suggesting the occurrence of a process of adaptation. The authors propose the existence of a dual light-perception system with at least two photoreceptors in , one promoting and one inhibiting conidiation.

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2004-02-01
2020-08-08
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References

  1. Arrach N., Fernández-Martín R., Cerdá-Olmedo E., Avalos J. 2001; A single gene for lycopene cyclase, phytoene synthase and regulation of carotene biosynthesis in Phycomyces . Proc Natl Acad Sci U S A98:1687–1692[CrossRef]
    [Google Scholar]
  2. Bergman K., Burke P. V., 8 other authors Cerdá-Olmedo E.. 1969; Phycomyces . Bacteriol Rev33:99–119
    [Google Scholar]
  3. Berrocal-Tito G., Sametz-Baron L., Eichenberg K., Horwitz B., Herrera-Estrella A. 1999; Rapid blue light regulation of a Trichoderma harzianum photolyase gene. J Biol Chem274:14288–14294[CrossRef]
    [Google Scholar]
  4. Betina V. 1984; Photoinduced conidiation in Trichoderma viride . Int J Microbiol2:55–68
    [Google Scholar]
  5. Betina V. 1995; Photoinduced conidiation in Trichoderma viride . Folia Microbiol49:219–224
    [Google Scholar]
  6. Briggs W. R., Christie J. M. 2002; Phototropins 1 and 2: versatile plant blue-light receptors. Trends Plant Sci7:204–210[CrossRef]
    [Google Scholar]
  7. Cerdá-Olmedo E., Corrochano L. 2001; Genetics of Phycomyces and its responses to light. In Photomovement vol. 1 pp589–620 Edited by Hader D. P., Lebert M.. Amsterdam: Elsevier;
    [Google Scholar]
  8. Corrochano L. M., Cerdá-Olmedo E. 1990; Photomorphogenesis in Phycomyces : competence period and stimulus-response relationships. J Photochem Photobiol B5:255–266[CrossRef]
    [Google Scholar]
  9. Corrochano L. M., Cerdá-Olmedo E. 1991; Photomorphogenesis in Phycomyces and other fungi. Photochem Photobiol45:319–327
    [Google Scholar]
  10. Corrochano L. M., Galland P., Lipson E. D., Cedá-Olmedo E. 1988; Photomorphogenesis in Phycomyces : fluence-response curves and action spectra. Planta174:315–320[CrossRef]
    [Google Scholar]
  11. De la Torre M., Cárdenas-Cota H. M. 1996; Production of Paecilomyces fumosoroseus conidia in submerged culture. Entomophaga41:443–453[CrossRef]
    [Google Scholar]
  12. Dunlap J. C. 1999; Molecular bases for circadian clocks. Cell96:271–280[CrossRef]
    [Google Scholar]
  13. Galagan J. E., Calvo S. E., Borkovich K. A.. 74 other authors 2003; Sequence of the filamentous fungus Neurospora crassa . Nature422:859–868[CrossRef]
    [Google Scholar]
  14. Galland P. 2002; Tropisms of Avena coleoptiles: sine law for gravitropism, exponential law for photogravitropic equilibrium. Planta215:779–784[CrossRef]
    [Google Scholar]
  15. Galland P., Lipson E. D. 1987; Blue light reception in Phycomyces phototropism: evidence for two photosystems operating in low and high intensity ranges. Proc Natl Acad Sci U S A84:104–108[CrossRef]
    [Google Scholar]
  16. Gressel J., Galun E. 1967; Morphogenesis in Trichoderma : photoinduction and RNA. Dev Biol15:575–598[CrossRef]
    [Google Scholar]
  17. Gyula P., Schäfer E., Nagy F. 2003; Light perception and signaling in higher plants. Curr Opin Plant Biol6:446–452[CrossRef]
    [Google Scholar]
  18. Harding R. W., Melles S. 1983; Genetic analysis of phototropism of Neurospora crassa perithecial beaks using white collar 1 and albino mutants. Plant Physiol72:996–1000[CrossRef]
    [Google Scholar]
  19. He Q., Cheng P., Yang Y., Wang L., Gardner K. H., Liu Y. 2002; White collar-1 binding transcription factor and light sensor. Science297:840–843[CrossRef]
    [Google Scholar]
  20. Horwitz B. A. 1984; The sensory pathway for photo-morphogenesis in Trichoderma and its mutants PhD thesis Weizmann Institute of Science;
    [Google Scholar]
  21. Jackson M. A., McGuire M. R., Lacey L. A., Wraigtht S. P. 1997; Liquid culture production of desiccation tolerant blastospores of the bioinsecticidal fungus Paecilomyces fumosoroseus . Mycol Res101:35–41[CrossRef]
    [Google Scholar]
  22. James R. R. 2001; Effects of exogenous nutrients on conidial germination and virulence against the silverleaf whitefly for two hyphomycetes. J Invertebr Pathol77:99–107[CrossRef]
    [Google Scholar]
  23. Kuhnel-Kratz C., Schafer J., Hader D. P. 1993; Phototaxis in the flagellate, Euglena gracilis , under the effect of microgravity. Microgravity Sci Technol6:188–193
    [Google Scholar]
  24. Kumagai T. 1978; Mycochrome system and conidial development in certain fungi imperfecti. Photochem Photobiol27:371–379[CrossRef]
    [Google Scholar]
  25. Kumagai T. 1988; Photocontrol of fungal development. Photochem Photobiol47:889–896[CrossRef]
    [Google Scholar]
  26. Kumagai T., Oda Y. 1969; An action spectrum for photoinduced sporulation in the fungus Trichoderma viride . Plant Cell Physiol10:387–392
    [Google Scholar]
  27. Lakin-Tomas P. L., Cote G. G., Brody S. 1990; Circadian rhythms in Neurospora crassa : biochemistry and genetics. Crit Rev Microbiol17:365–416[CrossRef]
    [Google Scholar]
  28. Lauter F. 1996; Molecular genetics of fungal photobiology. J Genet75:375–386[CrossRef]
    [Google Scholar]
  29. Lauter F., Russo V. 1991; Blue light induction of conidiation-specific genes in Neurospora crassa . Nucleic Acids Res19:6883–6886[CrossRef]
    [Google Scholar]
  30. Lauter F., Yamashiro C. T., Yanofsky C. 1997; Light stimulation of conidiation in Neurospora crassa : studies with the wild-type strain and mutans wc-1, wc-2 and acon-2 . J Photochem Photobiol B37:203–211[CrossRef]
    [Google Scholar]
  31. Linden H., Ballario P., Macino G. 1997; Blue light regulation in Neurospora crassa . Fungal Genet Biol22:141–150[CrossRef]
    [Google Scholar]
  32. Loros J. J., Dunlap J. C. 2001; Genetic and molecular analysis of circadian rhythms in Neurospora . Annu Rev Physiol63:757–794[CrossRef]
    [Google Scholar]
  33. Maier J., Hecker R., Rockel P., Ninnemann H. 2001; Role of nitric oxide synthase in light induced development of sporangiophores in Phycomyces blakesleeanus . Plant Physiol126:1323–1330[CrossRef]
    [Google Scholar]
  34. Mooney J. L., Yager L. N. 1990; Light is required for conidiation in Aspergillus nidulans . Genes Dev4:1473–1482[CrossRef]
    [Google Scholar]
  35. Ninnemann H. 1991; Photostimulation of conidiation of Neurospora crassa . J Photochem Photobiol B9:189–199[CrossRef]
    [Google Scholar]
  36. Pittendrigh C. S., Bruce B., Rosensweig N. S., Rubin M. L. 1959; Growth patterns in Neurospora crassa . Nature184:169–170[CrossRef]
    [Google Scholar]
  37. Potapova T. V., Levina N. N., Belozerscaya T. A., Kritsky M. S., Chailakhian L. M. 1984; Investigation of electrophysiological responses of Neurospora crassa to blue light. Arch Microbiol137:262–265[CrossRef]
    [Google Scholar]
  38. Qiu J., Hardin P. E. 1996; Developmental state and the circadian clock interact to influence the timing of eclosion in Drosophila melanogaster . J Biol Rhythms11:75–86[CrossRef]
    [Google Scholar]
  39. Rau W., Mitzka-Schnabel U. 1985; Carotenoid synthesis in Neurospora crassa . Methods Enzymol110:253–267
    [Google Scholar]
  40. Rua J., Rodriguez-Aparicio L. B., Busto F., Soler J. 1987; Effect of light on several metabolites of carbohydrate metabolism in Phycomyces blakesleeanus . J Bacteriol169:904–907
    [Google Scholar]
  41. Schrott E. L. 1980; Fluence response relationship of caretenogenesis in Neurospora crassa . Planta150:174–179[CrossRef]
    [Google Scholar]
  42. Schwerdtfeger C., Linden H. 2003; VIVID is a flavoprotein and serves as a fungal blue light photoreceptor for photoadaptation. EMBO J22:4846–4855[CrossRef]
    [Google Scholar]
  43. Sulová Z., Farkas V. 1991; Photoinduced conidiation in Trichoderma viride : a study with inhibitors. Folia Microbiol36:267–270[CrossRef]
    [Google Scholar]
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