1887

Abstract

The homologous and translocase operons of and pathogenic spp., respectively, are responsible for the translocation of anti-host effectors into the cytosol of infected eukaryotic cells. In , this operon is also required for -regulatory control. To probe for key molecular interactions during the infection process, the functional interchangeability of / and / was investigated. Secretion of PopB produced in a Δ null mutant of was only observed when co-produced with its native chaperone PcrH, but this was sufficient to complement the translocation defect. The Δ null mutant synthesized and secreted PopD even in the absence of native PcrH, yet this did not restore YopD-dependent -regulatory control or effector translocation. Thus, this suggests that key residues in YopD, which are not conserved in PopD, are essential for functional type III secretion.

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2003-09-01
2019-10-15
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