1887

Abstract

The effect of EDTA on serovar Typhimurium was studied in different growth phases with cells grown with or without Ca and Mg supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1--phenylnaphthylamine (a nonpolar hydrophobic probe, 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in involves a component that is independent of LPS release.

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2003-08-01
2019-09-18
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