1887

Abstract

Analysis of genomic clones encoding a putative laccase in homokaryon strains of led to the identification of an allelic variant of the previously described gene. A cDNA clone corresponding to this gene was expressed in and in . Enzyme assays and Western blots showed that both hosts secreted active laccase. Relative to the isozymic forms of the native enzyme, the -produced laccase had a higher molecular mass and gave a single band on IEF gels. In contrast, transformants secreted several isoforms remarkably similar to those of the native system. Considered together with previously reported Southern blots and protein sequencing, expression in supports the view that has a single laccase gene and that multiple isoforms result from post-translational processes. In addition, several lines of evidence strongly suggest that under copper limitation, secretes apoprotein which can be reconstituted by a short incubation with Cu(I) and to a lesser extent with Cu(II).

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2003-05-01
2019-11-19
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References

  1. Andersen, H. D., Jensen, E. B. & Welinder, K. G. ( 1992; ). A process for producing heme proteins. European Patent Application 0505311A2.
  2. Ando, K. ( 1970; ). Preparations and properties of apo- and reconstructed Rhus-laccases. J Biochem 68, 501–508.
    [Google Scholar]
  3. Berka, R. M., Schneider, P., Golightly, E. J., Brown, S. H., Madden, M., Brown, K. M., Halkier, T., Mondorf, K. & Xu, F. ( 1997; ). Characterization of the gene encoding an extracellular laccase of Myceliophthora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae. Appl Environ Microbiol 63, 3151–3157.
    [Google Scholar]
  4. Bligny, R., Gaillard, J. & Douce, R. ( 1986; ). Excretion of laccase by sycamore (Acer pseudoplatanus L.) cells. Effects of a copper deficiency. Biochem J 237, 583–588.
    [Google Scholar]
  5. Choi, G. H., Larson, T. G. & Nuss, D. L. ( 1992; ). Molecular analysis of the laccase gene from the chestnut blight fungus and selective suppression of its expression in an isogenic hypovirulent strain. Mol Plant-Microbe Interact 5, 119–128.[CrossRef]
    [Google Scholar]
  6. Cullen, D., Gray, G., Hayenga, K., Lamsa, M., Norton, S., Rey, M., Wilson, L. & Berka, R. ( 1987; ). Controlled expression and secretion of bovine chymosin by Aspergillus nidulans. Bio/Technology 5, 369–376.[CrossRef]
    [Google Scholar]
  7. Eggert, C., Temp, U. & Eriksson, K. ( 1997; ). Laccase is essential for lignin degradation by the white-rot fungus Pycnoporus cinnabarinus. FEBS Lett 407, 89–92.[CrossRef]
    [Google Scholar]
  8. Fukushima, Y. & Kirk, T. K. ( 1995; ). Laccase component of the Ceriporiopsis subvermispora lignin-degrading system. Appl Environ Microbiol 61, 872–876.
    [Google Scholar]
  9. Galhaup, C., Goller, S., Peterbauer, C. K., Strauss, J. & Haltrich, D. ( 2002; ). Characterization of the major laccase isoenzyme from Trametes pubescens and regulation of its synthesis by metal ions. Microbiology 148, 2159–2169.
    [Google Scholar]
  10. Geiger, J. P., Nicole, M., Nandris, D. & Rio, B. ( 1986; ). Root diseases of Hevea brasiliensis. Physiological and biochemical aspects of root aggregation. Phytochemistry 24, 2559–2561.
    [Google Scholar]
  11. Glenn, J. K. & Gold, M. H. ( 1985; ). Purification and characterization of an extracellular Mn(II)-dependent peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Arch Biochem Biophys 242, 329–341.[CrossRef]
    [Google Scholar]
  12. Hanna, P. M., McMillin, D. R., Pasenkiewicz-Gierula, M., Antholine, W. E. & Reinhammar, B. ( 1988; ). Type 2-depleted fungal laccase. Biochem J. 253, 561–568.
  13. Harrison, M. J., Nouwens, A. S., Jardine, D. R., Zachara, N. E., Gooley, A. A., Nevalainen, H. & Packer, N. H. ( 1998; ). Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei. Eur J Biochem 256, 119–127.[CrossRef]
    [Google Scholar]
  14. Hauenstein, B. L., Jr & McMillin, D. R. ( 1978; ). On the reconstitution of laccase from the Chinese lacquer tree. Biochem Biophys Res Commun 85, 505–510.[CrossRef]
    [Google Scholar]
  15. Horton, R., Hunt, H., Ho, S., Pullen, J. & Pease, L. ( 1989; ). Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77, 61–68.[CrossRef]
    [Google Scholar]
  16. Jonsson, L., Sjostrom, K., Haggstrom, I. & Nyman, P. O. ( 1995; ). Characterization of a laccase gene from the white-rot fungus Trametes versicolor and structural features of basidiomycete laccases. Biochim Biophys Acta 1251, 210–215.[CrossRef]
    [Google Scholar]
  17. Karahanian, E., Corsini, G., Lobos, S. & Vicuña, R. ( 1998; ). Structure and expression of a laccase gene from the ligninolytic basidiomycete Ceriporiopsis subvermispora. Biochim Biophys Acta 1443, 65–74.[CrossRef]
    [Google Scholar]
  18. Kersten, P. J., Witek, C., Vanden Wymelenberg, A. & Cullen, D. ( 1995; ). Phanerochaete chrysosporium glyoxal oxidase is encoded by two allelic variants: structure, genomic organization and heterologous expression of glx1 and glx2. J Bacteriol 177, 6106–6110.
    [Google Scholar]
  19. Kirk, T. K. & Farrell, R. L. ( 1987; ). Enzymatic “combustion”: the microbial degradation of lignin. Annu Rev Microbiol 41, 465–505.[CrossRef]
    [Google Scholar]
  20. Larrondo, L. F., Lobos, S., Stewart, P., Cullen, D. & Vicuña, R. ( 2001; ). Isoenzyme multiplicity and characterization of recombinant manganese peroxidases from Ceriporiopsis subvermispora and Phanerochaete chrysosporium. Appl Environ Microbiol 67, 2070–2075.[CrossRef]
    [Google Scholar]
  21. Leatham, G. & Stahman, M. A. ( 1981; ). Studies on the laccase of Lentinus edodes; specificity. localization and the development of fruiting bodies. J Gen Microbiol 125, 147–157.
    [Google Scholar]
  22. Lobos, S., Larrondo, L., Salas, L., Karahanian, E. & Vicuña, R. ( 1998; ). Cloning and molecular analysis of a cDNA and the Cs-mnp1 gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete Ceriporiopsis subvermispora. Gene 206, 185–193.[CrossRef]
    [Google Scholar]
  23. Mansur, M., Suarez, T., Fernandez-Larrea, J. B., Brizuela, M. A. & Gonzalez, A. E. ( 1997; ). Identification of a laccase gene family in the new lignin-degrading basidiomycete CECT 20197. Appl Environ Microbiol 63, 2637–2646.
    [Google Scholar]
  24. Marbach, I., Harel, E. & Mayer, A. M. ( 1985; ). Pectin, a second inducer for laccase production by Botrytis cinerea. Phytochemistry 24, 2559–2561.[CrossRef]
    [Google Scholar]
  25. Mayer, A. M. ( 1987; ). Polyphenol oxidases in plants – recent progress. Phytochemistry 26, 11–20.
    [Google Scholar]
  26. Omura, T. ( 1961; ). Studies on lacases of lacquer trees. Reconstitution of laccase from its protein and copper. J Biochem 50, 389–393.
    [Google Scholar]
  27. Pakula, T. M., Uusitalo, J., Saloheimo, M., Salonen, K., Aarts, R. J. & Penttila, M. ( 2000; ). Monitoring the kinetics of glycoprotein synthesis and secretion in the filamentous fungus Trichoderma reesei: cellobiohydrolase I (CBHI) as a model protein. Microbiology 146, 223–232.
    [Google Scholar]
  28. Palmieri, G., Giardina, P., Bianco, C., Fontanella, B. & Sannia, G. ( 2000; ). Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus. Appl Environ Microbiol 66, 920–924.[CrossRef]
    [Google Scholar]
  29. Paszczynski, A., Huynh, V.-B. & Crawford, R. L. ( 1985; ). Enzymatic activities of an extracellular, manganese-dependent peroxidase from Phanerochaete chrysosporium. FEMS Microbiol Lett 29, 37–41.[CrossRef]
    [Google Scholar]
  30. Perry, C., Smith, M., Britnell, C., Wood, D. & Thurston, C. ( 1993; ). Identification of two laccase genes in the cultivated mushroom Agaricus bisporus. J Gen Microbiol 139, 1209–1218.[CrossRef]
    [Google Scholar]
  31. Record, E., Punt, P. J., Chamkha, M., Labat, M., van Den Hondel, C. A. & Asther, M. ( 2002; ). Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme. Eur J Biochem 269, 602–609.[CrossRef]
    [Google Scholar]
  32. Reinhammer, B. ( 1984; ). In Copper Proteins and Copper Enzymes, pp. 1–35. Edited by R. Lontie. Boca Raton, FL: CRC Press.
  33. Salas, C., Lobos, S., Larrain, J., Salas, L., Cullen, D. & Vicuña, R. ( 1995; ). Properties of laccase isoenzymes produced by the basidiomycete Ceriporiopsis subvermispora. Biotechnol Appl Biochem 21, 323–333.
    [Google Scholar]
  34. Soden, D. M. & Dobson, A. D. ( 2001; ). Differential regulation of laccase gene expression in Pleurotus sajor-caju. Microbiology 147, 1755–1763.
    [Google Scholar]
  35. Stalbrand, H., Saloheimo, A., Vehmaanpera, J., Henrissat, B. & Penttila, M. ( 1995; ). Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain. Appl Environ Microbiol 61, 1090–1097.
    [Google Scholar]
  36. Tello, M., Seelenfreund, D., Lobos, S., Gaskell, J., Cullen, D. & Vicun∼a, R. ( 2001; ). Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora. FEMS Microbiol Lett 199, 91–96.[CrossRef]
    [Google Scholar]
  37. Wahleithmer, J. A., Xu, F., Brown, K., Brown, S., Golightly, E., Halkier, T., Kauppinen, S., Pederson, A. & Schneider, P. ( 1995; ). The identification and characterization of four laccase genes from the plant pathogenic fungus Rhizoctonia solani. Curr Genet 29, 395–403.
    [Google Scholar]
  38. Yaver, D. & Golightly, E. ( 1996; ). Cloning and characterization of three laccase genes from the white-rot basidiomycete Trametes villosa: genomic organization of the laccase gene family. Gene 181, 95–102.[CrossRef]
    [Google Scholar]
  39. Yaver, D., Xu, F., Golightly, E. & 7 other authors ( 1996; ). The purification, characterization, molecular cloning and expression of two laccase genes from the white-rot basidiomycete Trametes villosa. Appl Environ Microbiol 62, 834–841.
    [Google Scholar]
  40. Yaver, D. S., Overjero, M. D., Xu, F., Nelson, B. A., Brown, K. M., Halkier, T., Bernauer, S., Brown, S. H. & Kauppinen, S. ( 1999; ). Molecular characterization of laccase genes from the basidiomycete Coprinus cinereus and heterologous expression of the laccase lcc1. Appl Environ Microbiol 65, 4943–4948.
    [Google Scholar]
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