1887

Abstract

, unlike , was able to use extracellular haemin as an iron source. Haemin uptake kinetics by cells showed two phases: a rapid phase of haemin binding (with a of about 0·2 μM) followed by a slower uptake phase. Both phases were strongly induced in iron-deficient cells compared to iron-rich cells. Haemin uptake did not depend on the previously characterized reductive iron uptake system and siderophore uptake system. , encoding a putative haem oxygenase, was shown to be required for iron assimilation from haemin. A double Δ mutant was constructed. This mutant could not grow with haemin as the sole iron source, although haemin uptake was not affected. The three different iron uptake systems (reductive, siderophore and haemin) were regulated independently and in a complex manner. expression was induced by iron deprivation, by haemin and by a shift of temperature from 30 to 37 °C. expression was strongly deregulated in a Δ mutant but not in a Δ mutant. colonies forming on agar plates with haemin as the sole iron source showed a very unusual morphology. Colonies were made up of tubular structures that were organized into a complex network. The effect of haemin on filamentation was increased in the double Δ mutant. This study provides the first experimental evidence that haem oxygenase is required for iron assimilation from haem by a pathogenic fungus.

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2003-03-01
2022-01-29
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