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Microbiology Society logo Microbiology

Volume 154, Issue 11

Carbon source-dependent modulation of NADP-glutamate dehydrogenases in isophthalate-degrading strain PP4, strain PPD and strain ISP4 Free

Abstract

strain ISP4 metabolizes isophthalate rapidly compared with strain PP4 and strain PPD. Isophthalate has been reported to be a potent competitive inhibitor of glutamate dehydrogenase (GDH). Exogenous supplementation of isophthalate with glutamate or -ketoglutarate at 1 mM concentration caused strains PP4 and PPD to grow faster than in the presence of isophthalate alone; however, no such effect was observed in strain ISP4. When grown on isophthalate, all strains showed activity of NADP-dependent GDH (NADP-GDH), while cells grown on glucose, 2× yeast extract-tryptone broth (2YT) or glutamate showed activities of both NAD-dependent GDH (NAD-GDH) and NADP-GDH. Activity staining, inhibition and thermal stability studies indicated the carbon source-dependent presence of two (GDH and GDH), three (GDH, GDH and GDH) and one (GDH) forms of NADP-GDH in strains PP4, PPD and ISP4, respectively. The results demonstrate the carbon source-dependent modulation of different forms of NADP-GDH in these bacterial strains. This modulation may help the efficient utilization of isophthalate as a carbon source by overcoming the inhibitory effect on GDH.

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Keyword(s): GDH, glutamate dehydrogenase , mHB, m-hydroxybenzoate , NAD-GDH, NAD-dependent GDH , NADP-GDH, NADP-dependent GDH , pHB, p-hydroxybenzoate and α-KG, α-ketoglutarate
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2008-11-01
2025-04-30
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/content/journal/micro/10.1099/mic.0.2008/022087-0
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Carbon source-dependent modulation of NADP-glutamate dehydrogenases in isophthalate-degrading Pseudomonas aeruginosa strain PP4, Pseudomonas strain PPD and Acinetobacter lwoffii strain ISP4
Microbiology 154, 3329 (2008); https://doi.org/10.1099/mic.0.2008/022087-0
/content/journal/micro/10.1099/mic.0.2008/022087-0
/content/journal/micro/10.1099/mic.0.2008/022087-0
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