1887

Abstract

We cloned a DNA fragment that suppressed the aerial-mycelium-deficient phenotype in an mutant of when it was introduced into the cells via a high-copy-number plasmid. The gene cluster was identified as being responsible for this suppressive activity. The proteins encoded by were of unknown function, but the operon structure was found to be conserved in all the strains of spp. and related organisms whose genomes have been sequenced. , the flanking opposite coding sequence, encoded a putative DNA-binding protein. Subcloning revealed that the presence of all five coding sequences was essential for complete suppression. Scanning electron microscopy of strains carrying the gene cluster at a high copy-number revealed that bundle-like structures consisting of several aerial hyphae were often formed. S1 nuclease protection analyses were performed to identify the transcriptional start site in the promoters preceding and . The promoter preceding was highly active during vegetative growth. Null mutants for among the and A3(2) cells exhibited bald phenotypes; this suggested a positive regulatory role of this gene cluster in the onset of morphogenesis in these two phylogenetically distinct species.

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2008-12-01
2024-04-26
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