1887

Abstract

We cloned a DNA fragment that suppressed the aerial-mycelium-deficient phenotype in an mutant of when it was introduced into the cells via a high-copy-number plasmid. The gene cluster was identified as being responsible for this suppressive activity. The proteins encoded by were of unknown function, but the operon structure was found to be conserved in all the strains of spp. and related organisms whose genomes have been sequenced. , the flanking opposite coding sequence, encoded a putative DNA-binding protein. Subcloning revealed that the presence of all five coding sequences was essential for complete suppression. Scanning electron microscopy of strains carrying the gene cluster at a high copy-number revealed that bundle-like structures consisting of several aerial hyphae were often formed. S1 nuclease protection analyses were performed to identify the transcriptional start site in the promoters preceding and . The promoter preceding was highly active during vegetative growth. Null mutants for among the and A3(2) cells exhibited bald phenotypes; this suggested a positive regulatory role of this gene cluster in the onset of morphogenesis in these two phylogenetically distinct species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2008/021535-0
2008-12-01
2019-11-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/154/12/3668.html?itemId=/content/journal/micro/10.1099/mic.0.2008/021535-0&mimeType=html&fmt=ahah

References

  1. Chater, K. F. ( 1993; ). Genetics of differentiation in Streptomyces. Annu Rev Microbiol 47, 685–713.[CrossRef]
    [Google Scholar]
  2. Gregory, M. A., Till, R. & Smith, M. C. ( 2003; ). Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors. J Bacteriol 185, 5320–5323.[CrossRef]
    [Google Scholar]
  3. Gust, B., Challis, G. L., Fowler, K., Kieser, T. & Chater, K. F. ( 2003; ). PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci U S A 100, 1541–1546.[CrossRef]
    [Google Scholar]
  4. Horinouchi, S. ( 2007; ). Mining and polishing of the treasure trove in the bacterial genus Streptomyces. Biosci Biotechnol Biochem 71, 283–299.[CrossRef]
    [Google Scholar]
  5. Katz, E., Thompson, C. J. & Hopwood, D. A. ( 1983; ). Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol 129, 2703–2714.
    [Google Scholar]
  6. Kelemen, G. H., Viollier, P. H., Tenor, J., Marri, L., Buttner, M. J. & Thompson, C. J. ( 2001; ). A connection between stress and development in the multicellular prokaryote Streptomyces coelicolor A3(2). Mol Microbiol 40, 804–814.[CrossRef]
    [Google Scholar]
  7. Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K. F. & Hopwood, D. A. ( 2000; ). Practical Streptomyces Genetics. Norwich, UK: John Innes Foundation.
  8. Maniatis, T., Fritsch, E. F. & Sambrook, J. ( 1982; ). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  9. Ohnishi, Y., Kameyama, S., Onaka, H. & Horinouchi, S. ( 1999; ). The A-factor regulatory cascade leading to streptomycin biosynthesis in Streptomyces griseus: identification of a target gene of the A-factor receptor. Mol Microbiol 34, 102–111.[CrossRef]
    [Google Scholar]
  10. Ohnishi, Y., Ishikawa, J., Hara, H., Suzuki, H., Ikenoya, M., Ikeda, H., Yamashita, A., Hattori, M. & Horinouchi, S. ( 2008; ). Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. J Bacteriol 190, 4050–4060.[CrossRef]
    [Google Scholar]
  11. Shinkawa, H., Hatada, Y., Okada, M., Kinashi, H. & Nimi, O. ( 1995; ). Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus. J Biochem 118, 494–499.
    [Google Scholar]
  12. Sollner-Webb, B. & Reeder, R. H. ( 1979; ). The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis. Cell 18, 485–499.[CrossRef]
    [Google Scholar]
  13. Song, J., Tyler, R. C., Lee, M. S., Tyler, E. M. & Markley, J. L. ( 2005; ). Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex. J Mol Biol 354, 1043–1051.[CrossRef]
    [Google Scholar]
  14. Sorensen, K. I. & Hove-Jensen, B. ( 1996; ). Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression. J Bacteriol 178, 1003–1011.
    [Google Scholar]
  15. Stephens, K., Hartzell, P. & Kaiser, D. ( 1989; ). Gliding motility in Myxococcus xanthus: mgl locus, RNA, and predicted protein products. J Bacteriol 171, 819–830.
    [Google Scholar]
  16. Ueda, K., Hsheh, C. W., Tosaki, T., Shinkawa, H., Beppu, T. & Horinouchi, S. ( 1998; ). Characterization of an A-factor-responsive repressor for amfR essential for onset of aerial mycelium formation in Streptomyces griseus. J Bacteriol 180, 5085–5093.
    [Google Scholar]
  17. Ueda, K., Oinuma, K., Ikeda, G., Hosono, K., Ohnishi, Y., Horinouchi, S. & Beppu, T. ( 2002; ). AmfS, an extracellular peptidic morphogen in Streptomyces griseus. J Bacteriol 184, 1488–1492.[CrossRef]
    [Google Scholar]
  18. Ueda, K., Takano, H., Nishimoto, M., Inaba, H. & Beppu, T. ( 2005; ). Dual transcriptional control of amfTSBA, which regulates the onset of cellular differentiation in Streptomyces griseus. J Bacteriol 187, 135–142.[CrossRef]
    [Google Scholar]
  19. Willey, J. M., Willems, A., Kodani, S. & Nodwell, J. R. ( 2006; ). Morphogenetic surfactants and their role in the formation of aerial hyphae in Streptomyces coelicolor. Mol Microbiol 59, 731–742.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2008/021535-0
Loading
/content/journal/micro/10.1099/mic.0.2008/021535-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error