1887

Abstract

Enteropathogenic (EPEC) adheres and to epithelial cells. Two main adhesins, the bundle-forming pilus and intimin, encoded by the operon and , respectively, are responsible for the localized and the intimate adherence phenotypes. Deletion of the operon of EPEC abolishes the transport of inorganic phosphate through the phosphate-specific transport system and causes the constitutive expression of the PHO regulon genes. In the absence of there is a decrease in the expression of the main EPEC adhesins and a reduction in bacterial adherence to epithelial cells . This effect is not related to PHO constitutivity, because a Δ double mutant that is defective in the transcription of the PHO genes also displayed low levels of adherence and expression of adhesins. Likewise, a PHO-constitutive mutation did not affect bacterial adherence. The expression of the operon, which encodes the and regulators PerA and PerC, is also negatively affected by the deletion. Overall, the data presented here demonstrate that the operon of EPEC plays a positive role in the bacterial adherence mechanism by increasing the expression of and and consequently the transcription of and .

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2008-07-01
2020-01-25
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