1887

Abstract

Some strains produce a catechol-type siderophore named vanchrobactin, whose biosynthetic pathway has not been completely elucidated. In addition to the previously described genes , , , , and , in the present study we have identified the genes encoding a DAHP (3-deoxy--arabino-heptulosonate-7-phosphate) synthetase (), a phosphopantheteinyl transferase (), a LysR-family transcriptional regulator () and a putative siderophore receptor (). A deletion affecting or greatly reduced growth under iron-limiting conditions, whereas deletion of did not have significant effects. Vanchrobactin production was abolished in the mutant, whereas the mutant retained a residual vanchrobactin production ability. Reverse transcriptase-mediated PCR indicated that this 11-gene cluster is organized into six iron-regulated transcriptional units. Transcriptional fusions demonstrated that the ferric uptake regulator (Fur) protein is the main iron-responsive regulator of these genes. Interestingly, the gene was strongly iron-repressed, but Fur was not essential for this repression. In addition, the maximal expression from the promoter was achieved only in the presence of an intact copy of . Analysis of the -galactosidase activities of a  : :  fusion in a mutant and in the presence of added vanchrobactin suggested that a ferric-vanchrobactin-dependent activator plays a positive regulatory role in transcription of the operon. This possibility is reinforced by the presence of a predicted AraC box upstream of . We propose that vanchrobactin biosynthesis is subjected to a complex regulatory circuitry aimed at adjusting vanchrobactin production for the maintenance of iron homeostasis in .

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2008-05-01
2021-10-25
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