1887

Abstract

Recent data from microarray analysis have shown that integrated prophages are the most frequent sources of genomic variation between different strains of serovar Typhimurium (. Typhimurium). This led us to hypothesize that PCR detection of the integrated prophages might be an efficient typing tool that could be used as an alternative to PFGE. In this study, we optimized four triplex PCRs specific for 12 target sequences of mostly prophage origin, and tested them in 102 field strains. The same set of strains was also characterized by PFGE. Among the strains, 22 different multiplex PCR, and 25 different PFGE profiles, were identified. Despite the fact that the PFGE was slightly more discriminatory, multiplex PCR typing, owing to its simplicity and potential of simple data sharing between laboratories, represents an interesting user-friendly alternative to PFGE typing of . Typhimurium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2007/015156-0
2008-05-01
2020-07-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/154/5/1384.html?itemId=/content/journal/micro/10.1099/mic.0.2007/015156-0&mimeType=html&fmt=ahah

References

  1. Anderson E. S., Ward L. R., Saxe M. J., de Sa J. D.. 1977; Bacteriophage-typing designations of Salmonella typhimurium . J Hyg (Lond)78:297–300
    [Google Scholar]
  2. Faldynova M., Pravcova M., Sisak F., Havlickova H., Kolackova I., Cizek A., Karpiskova R., Rychlik I.. 2003; Evolution of antibiotic resistance in Salmonella enterica serovar Typhimurium strains isolated in the Czech Republic between 1984 and 2002. Antimicrob Agents Chemother47:2002–2005
    [Google Scholar]
  3. Galanis E., Lo Fo Wong D. M., Patrick M. E., Binsztein N., Cieslik A., Chalermchikit T., Aidara-Kane A., Ellis A., Angulo F. J., Wegener H. C.. 2006; Web-based surveillance and global Salmonella distribution, 2000–2002. Emerg Infect Dis12:381–388
    [Google Scholar]
  4. Hermans A. P., Abee T., Zwietering M. H., Aarts H. J.. 2005; Identification of novel Salmonella enterica serovar Typhimurium DT104-specific prophage and nonprophage chromosomal sequences among serovar Typhimurium isolates by genomic subtractive hybridization. Appl Environ Microbiol71:4979–4985
    [Google Scholar]
  5. Hradecka H., Kolackova I., Karpiskova R., Rychlik I.. 2006; An outbreak of human salmonellosis caused by ampicillin-resistant Salmonella enterica serovar Enteritidis PT13 in the Czech Republic. Epidemiol Infect134:737–740
    [Google Scholar]
  6. Hunter S. B., Vauterin P., Lambert-Fair M. A., Van Duyne M. S., Kubota K., Graves L., Wrigley D., Barrett T., Ribot E.. 2005; Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J Clin Microbiol43:1045–1050
    [Google Scholar]
  7. Matiasovicova J., Faldynova M., Pravcova M., Karpiskova R., Kolackova I., Damborsky J., Rychlik I.. 2003; Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium. FEMS Microbiol Lett223:281–286
    [Google Scholar]
  8. Matiasovicova J., Adams P., Barrow P. A., Hradecka H., Malcova M., Karpiskova R., Budinska E., Pilousova L., Rychlik I.. 2007; Identification of putative ancestors of the multidrug-resistant Salmonella enterica serovar Typhimurium DT104 clone harboring the Salmonella genomic island 1. Arch Microbiol187:415–424
    [Google Scholar]
  9. McClelland M., Sanderson K. E., Spieth J., Clifton S. W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M.. other authors 2001; Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature413:852–856
    [Google Scholar]
  10. Mikasova E., Drahovska H., Szemes T., Kuchta T., Karpiskova R., Sasik M., Turna J.. 2005; Characterization of Salmonella enterica serovar Typhimurium strains of veterinary origin by molecular typing methods. Vet Microbiol109:113–120
    [Google Scholar]
  11. Mirold S., Rabsch W., Rohde M., Stender S., Tschape H., Russmann H., Igwe E., Hardt W. D.. 1999; Isolation of a temperate bacteriophage encoding the type III effector protein SopE from an epidemic Salmonella typhimurium strain. Proc Natl Acad Sci U S A96:9845–9850
    [Google Scholar]
  12. Porwollik S., Boyd E. F., Choy C., Cheng P., Florea L., Proctor E., McClelland M.. 2004; Characterization of Salmonella enterica subspecies I genovars by use of microarrays. J Bacteriol186:5883–5898
    [Google Scholar]
  13. Reen F. J., Boyd E. F., Porwollik S., Murphy B. P., Gilroy D., Fanning S., McClelland M.. 2005; Genomic comparisons of Salmonella enterica serovar Dublin, Agona, and Typhimurium strains recently isolated from milk filters and bovine samples from Ireland, using a Salmonella microarray. Appl Environ Microbiol71:1616–1625
    [Google Scholar]
  14. Ross I. L., Heuzenroeder M. W.. 2005; Discrimination within phenotypically closely related definitive types of Salmonella enterica serovar Typhimurium by the multiple amplification of phage locus typing technique. J Clin Microbiol43:1604–1611
    [Google Scholar]
  15. Van Immerseel F., Pasmans F., De Buck J., Rychlik I., Hradecka H., Collard J. M., Wildemauwe C., Heyndrickx M., Ducatelle R., Haesebrouck F.. 2004; Cats as a risk for transmission of antimicrobial drug-resistant Salmonella . Emerg Infect Dis10:2169–2174
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2007/015156-0
Loading
/content/journal/micro/10.1099/mic.0.2007/015156-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error