1887

Abstract

Golgi equivalents (GEs) process materials in the fungal secretory pathway. Despite the importance of localized secretion in fungal tip growth, GE behaviour in living hyphae has not been documented. The distribution was monitored of an putative GE-associated protein, CopA, tagged with GFP (CopA–GFP). This co-localized with a Golgi body/GE marker established in other systems, -2,6-sialyltransferase, tagged with red fluorescent protein (ST–RFP). CopA–GFP and ST–RFP distributions responded similarly to brefeldin A, which impairs Golgi/GE trafficking. We used a CopA–GFP, strain to study GE distribution and behaviour in growing hyphae. This strain has a wild-type phenotype at 28 °C, can be manipulated by changing growth temperature or by use of cytoskeleton inhibitors, and its GE behaviour is consistent with that in a wild-type-morphology strain. GEs were more abundant at hyphal tips than subapically, and showed saltatory motility in all directions. Anterograde GE movements predominated. These were positively correlated with, but at least 10-fold faster than, hyphal growth rate, under all growth and experimental conditions investigated. The actin inhibitor latrunculin B reduced both anterograde GE movement and hyphal growth rate, whereas the microtubule (MT) depolymerizer benomyl increased anterograde GE movement and decreased hyphal growth rate. The MT stabilizer taxol increased GE movement but not hyphal growth rate. GE motility appears to have a complex dependence on both actin and MTs. We present a model for apical delivery of growth materials in which GEs play a role in long-distance transport.

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2008-05-01
2019-10-17
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