1887

Abstract

A homologous gene () of a putative nucleoside hydrolase (NH), which had been identified from the exosporia of and spores, was cloned from subsp. . Disruption of did not affect the vegetative growth and sporulation of , but promoted both inosine- and adenosine-induced spore germination. The inosine- or adenosine-induced germination rate decreased when the wild-type gene was overexpressed in . The gene product was characterized as a purine-specific NH. The kinetic parameters of IunH with inosine as substrate were =399±115 M, =48.9±8.5 s and / =1.23×10 M s. The optimal pH and temperature for IunH were found to be pH 6 and 80 °C. Meanwhile, the specific activity of inosine hydrolase in intact spores of the wild-type strain with inosine as substrate was 2.89±0.23×10 μmol min (mg dry wt). These results indicate that IunH is important in moderating inosine- or adenosine-induced germination of spores.

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2008-05-01
2019-11-15
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