1887

Abstract

A homologous gene () of a putative nucleoside hydrolase (NH), which had been identified from the exosporia of and spores, was cloned from subsp. . Disruption of did not affect the vegetative growth and sporulation of , but promoted both inosine- and adenosine-induced spore germination. The inosine- or adenosine-induced germination rate decreased when the wild-type gene was overexpressed in . The gene product was characterized as a purine-specific NH. The kinetic parameters of IunH with inosine as substrate were =399±115 M, =48.9±8.5 s and / =1.23×10 M s. The optimal pH and temperature for IunH were found to be pH 6 and 80 °C. Meanwhile, the specific activity of inosine hydrolase in intact spores of the wild-type strain with inosine as substrate was 2.89±0.23×10 μmol min (mg dry wt). These results indicate that IunH is important in moderating inosine- or adenosine-induced germination of spores.

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2008-05-01
2020-08-08
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References

  1. Boydston J. A., Yue L., Kearney J. F., Turnbough C. L. Jr. 2006; The ExsY protein is required for complete formation of the exosporium of Bacillus anthracis . J Bacteriol188:7440–7448
    [Google Scholar]
  2. Challacombe J. F., Altherr M. R., Xie G., Bhotika S. S., Brown N., Bruce D., Campbell C. S., Campbell M. L., Chen J.. other authors 2007; The complete genome sequence of Bacillus thuringiensis Al Hakam. J Bacteriol189:3680–3681
    [Google Scholar]
  3. Crickmore N.. 2006; Beyond the spore – past and future developments of Bacillus thuringiensis as a biopesticide. J Appl Microbiol101:616–619
    [Google Scholar]
  4. Foerster H. F., Foster J. W.. 1966; Response of Bacillus spores to combinations of germinative compounds. J Bacteriol91:1168–1177
    [Google Scholar]
  5. Giabbai B., Degano M.. 2004; Crystal structure to 1.7 Å of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy. Structure12:739–749
    [Google Scholar]
  6. Gonzalez J. M. Jr, Dulmage H. T., Carlton B. C.. 1981; Correlation between specific plasmids and delta-endotoxin production in Bacillus thuringiensis . Plasmid5:352–365
    [Google Scholar]
  7. Gopaul D. N., Meyer S. L., Degano M., Sacchettini J. C., Schramm V. L.. 1996; Inosine-uridine nucleoside hydrolase from Crithidia fasciculata . Genetic characterization, crystallization, and identification of histidine 241 as a catalytic site residue. Biochemistry35:5963–5970
    [Google Scholar]
  8. Helgason E., Okstad O. A., Caugant D. A., Johansen H. A., Fouet A., Mock M., Hegna I., Kolsto A. B.. 2000; Bacillus anthracis , Bacillus cereus , and Bacillus thuringiensis – one species on the basis of genetic evidence. Appl Environ Microbiol66:2627–2630
    [Google Scholar]
  9. Hills G. M.. 1949; Chemical factors in the germination of spore-bearing aerobes; the effect of yeast extract on the germination of Bacillus anthracis and its replacement by adenosine. Biochem J45:353–362
    [Google Scholar]
  10. Hornstra L. M., de Vries Y. P., de Vos W. M., Abee T., Wells-Bennik M. H.. 2005; gerR , a novel ger operon involved in l-alanine- and inosine-initiated germination of Bacillus cereus ATCC 14579. Appl Environ Microbiol71:774–781
    [Google Scholar]
  11. Hornstra L. M., de Vries Y. P., Wells-Bennik M. H., de Vos W. M., Abee T.. 2006; Characterization of germination receptors of Bacillus cereus ATCC 14579. Appl Environ Microbiol72:44–53
    [Google Scholar]
  12. Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N.. other authors 2003; Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis . Nature423:87–91
    [Google Scholar]
  13. Lawrence N. L.. 1955; The cleavage of adenosine by spores of Bacillus cereus . J Bacteriol70:577–582
    [Google Scholar]
  14. Lereclus D., Arantes O., Chaufaux J., Lecadet M.. 1989; Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis . FEMS Microbiol Lett51:211–217
    [Google Scholar]
  15. Liu Y. B., Tabashnik B. E., Moar W. J., Smith R. A.. 1998; Synergism between Bacillus thuringiensis spores and toxins against resistant and susceptible diamondback moths ( Plutella xylostella . Appl Environ Microbiol64:1385–1389
    [Google Scholar]
  16. Matz L. L., Beaman T. C., Gerhardt P.. 1970; Chemical composition of exosporium from spores of Bacillus cereus . J Bacteriol101:196–201
    [Google Scholar]
  17. Parkin D. W.. 1996; Purine-specific nucleoside N -ribohydrolase from Trypanosoma brucei brucei . Purification, specificity, and kinetic mechanism. J Biol Chem271:21713–21719
    [Google Scholar]
  18. Parkin D. W., Horenstein B. A., Abdulah D. R., Estupinan B., Schramm V. L.. 1991; Nucleoside hydrolase from Crithidia fasciculata . Metabolic role, purification, specificity, and kinetic mechanism. J Biol Chem266:20658–20665
    [Google Scholar]
  19. Pelle R., Schramm V. L., Parkin D. W.. 1998; Molecular cloning and expression of a purine-specific N -ribohydrolase from Trypanosoma brucei brucei . Sequence, expression, and molecular analysis. J Biol Chem273:2118–2126
    [Google Scholar]
  20. Ramarao N., Lereclus D.. 2005; The InhA1 metalloprotease allows spores of the B. cereus group to escape macrophages. Cell Microbiol7:1357–1364
    [Google Scholar]
  21. Rasko D. A., Ravel J., Okstad O. A., Helgason E., Cer R. Z., Jiang L., Shores K. A., Fouts D. E., Tourasse N. J.. other authors 2004; The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1. Nucleic Acids Res32:977–988
    [Google Scholar]
  22. Redmond C., Baillie L. W., Hibbs S., Moir A. J., Moir A.. 2004; Identification of proteins in the exosporium of Bacillus anthracis . Microbiology150:355–363
    [Google Scholar]
  23. Ribeiro J. M., Valenzuela J. G.. 2003; The salivary purine nucleosidase of the mosquito, Aedes aegypti . Insect Biochem Mol Biol33:13–22
    [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold spring Harbor Laboratory;
    [Google Scholar]
  25. Schnepf E., Crickmore N., Van Rie J., Lereclus D., Baum J., Feitelson J., Zeigler D. R., Dean D. H.. 1998; Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev62:775–806
    [Google Scholar]
  26. Setlow P.. 2003; Spore germination. Curr Opin Microbiol6:550–556
    [Google Scholar]
  27. Smith K., Youngman P.. 1992; Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spoIIM gene. Biochimie74:705–711
    [Google Scholar]
  28. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C.. 1985; Measurement of protein using bicinchoninic acid. Anal Biochem150:76–85
    [Google Scholar]
  29. Steichen C., Chen P., Kearney J. F., Turnbough C. L. Jr. 2003; Identification of the immunodominant protein and other proteins of the Bacillus anthracis exosporium. J Bacteriol185:1903–1910
    [Google Scholar]
  30. Steichen C. T., Kearney J. F., Turnbough C. L. Jr. 2005; Characterization of the exosporium basal layer protein BxpB of Bacillus anthracis . J Bacteriol187:5868–5876
    [Google Scholar]
  31. Stewart B. T., Halvorson H. O.. 1953; Studies on the spores of aerobic bacteria. I. The occurrence of alanine racemase. J Bacteriol65:160–166
    [Google Scholar]
  32. Todd S. J., Moir A. J., Johnson M. J., Moir A.. 2003; Genes of Bacillus cereus and Bacillus anthracis encoding proteins of the exosporium. J Bacteriol185:3373–3378
    [Google Scholar]
  33. Versees W., Decanniere K., Pelle R., Depoorter J., Brosens E., Parkin D. W., Steyaert J.. 2001; Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax . J Mol Biol307:1363–1379
    [Google Scholar]
  34. Yan X., Gai Y., Liang L., Liu G., Tan H.. 2007; A gene encoding alanine racemase is involved in spore germination in Bacillus thuringiensis . Arch Microbiol187:371–378
    [Google Scholar]
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