1887

Abstract

YapC, a putative autotransporter protein, shows strong homology to the enterotoxigenic adhesin TibA. As a potentially important surface protein of , we analysed YapC for several activities. When expressed in the non-pathogenic Fim strain AAEC185, YapC mediated attachment to both murine-derived macrophage-like cells (RAW264.7) and human-derived epithelial-like cells (HEp-2). In addition, expression of YapC on the surface of led to autoaggregation in DMEM tissue culture medium, a phenomenon associated with virulence in species. YapC also mediated formation of biofilm-like deposits by AAEC185. Deletion of in strain KIM5 resulted in no change in adhesion to either RAW264.7 or HEp-2 cells, or in biofilm formation. Lack of a phenotype for the Δ mutant may reflect the relatively low level of expression , as assessed by RT-PCR, and/or redundant functions expressed . These data demonstrate several activities for YapC that may function during infection.

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2008-06-01
2019-09-21
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