1887

Abstract

Clumping factor B (ClfB) of binds to cytokeratin 10 and to fibrinogen. In this study the binding site in human fibrinogen was localized to a short region within the C terminus of the A-chain. ClfB only bound to the A-chain of fibrinogen in a ligand-affinity blot and in solid-phase assays with purified recombinant fibrinogen chains. A variant of fibrinogen with wild-type B- and -chains but with a deletion that lacked the C-terminal residues from 252–610 of the A-chain did not support adherence of Newman expressing ClfB. A series of truncated mutants of the recombinant A-chain were tested for their ability to support adherence of Newman ClfB, which allowed the binding site to be localized to a short segment of the unfolded flexible repeated sequence within the C terminus of the A-chain. This was confirmed by two amino acid substititions within repeat 5 of the recombinant A-chain which did not support adherence of Newman ClfB. expressing ClfB mutants with amino acid substitutions (N256 and Q235) located in the putative ligand-binding trench between domains N2 and N3 of the A-domain were defective in adherence to immobilized fibrinogen and cytokeratin 10, suggesting that both ligands bind to the same or overlapping regions.

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2008-02-01
2020-07-11
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References

  1. Bolyard M. G., Lord S. T.. 1988; High-level expression of a functional human fibrinogen gamma chain in Escherichia coli . Gene66:183–192
    [Google Scholar]
  2. Burton R. A., Tsurupa G., Medved L., Tjandra N.. 2006; Identification of an ordered compact structure within the recombinant bovine fibrinogen alphaC-domain fragment by NMR. Biochemistry45:2257–2266
    [Google Scholar]
  3. Cierniewski C. S., Budzynski A. Z.. 1992; Involvement of the alpha-chain in fibrin clot formation. Effect of monoclonal-antibodies. Biochemistry31:4248–4253
    [Google Scholar]
  4. Cole A. M., Tahk S., Oren A., Yoshioka D., Kim Y. H., Park A., Ganz T.. 2001; Determinants of Staphylococcus aureus nasal carriage. Clin Diagn Lab Immunol8:1064–1069
    [Google Scholar]
  5. Credo R. B., Curtis C. G., Lorand L.. 1981; Alpha-chain domain of fibrinogen controls generation of fibrinoligase (coagulation factor XIIIa. Calcium ion regulatory aspects. Biochemistry20:3770–3778
    [Google Scholar]
  6. Davis S. L., Gurusiddappa S., McCrea K. W., Perkins S., Hook M.. 2001; SdrG, a fibrinogen-binding bacterial adhesin of the microbial surface components recognizing adhesive matrix molecules subfamily from Staphylococcus epidermidis , targets the thrombin cleavage site in the Bbeta chain. J Biol Chem276:27799–27805
    [Google Scholar]
  7. Doolittle R. F.. 1984; Fibrinogen and fibrin. Annu Rev Biochem53:195–229
    [Google Scholar]
  8. Duthie E. S., Lorenz L. L.. 1952; Staphylococcal coagulase; mode of action and antigenicity. J Gen Microbiol6:95–107
    [Google Scholar]
  9. Gorkun O. V., Veklich Y. I., Medved L. V., Henschen A. H., Weisel J. W.. 1994; Role of the alpha C domains of fibrin in clot formation. Biochemistry33:6986–6997
    [Google Scholar]
  10. Gorkun O. V., Henschen-Edman A. H., Ping L. F., Lord S. T.. 1998; Analysis of A alpha 251 fibrinogen: the alpha C domain has a role in polymerization, albeit more subtle than anticipated from the analogous proteolytic fragment X. Biochemistry37:15434–15441
    [Google Scholar]
  11. Henschen A., McDonagh J.. 1986; In Fibrinogen, Fibrin and Factor XIII in Blood Coagulation pp171–241 Edited by Zwaal R. F. A., Hemker H. C. Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  12. Herrick S., Blanc-Brude O., Gray A., Laurent G.. 1999; Fibrinogen. Int J Biochem Cell Biol31:741–746
    [Google Scholar]
  13. Jonsson K., McDevitt D., McGavin M. H., Patti J. M., Hook M.. 1995; Staphylococcus aureus expresses a major histocompatibility complex class II analog. J Biol Chem270:21457–21460
    [Google Scholar]
  14. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685
    [Google Scholar]
  15. Lord S. T.. 1985; Expression of a cloned human fibrinogen cDNA in Escherichia coli : synthesis of an A alpha polypeptide. DNA4:33–38
    [Google Scholar]
  16. Mazmanian S. K., Liu G., Ton-That H., Schneewind O.. 1999; Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science285:760–763
    [Google Scholar]
  17. McAleese F. M., Walsh E. J., Sieprawska M., Potempa J., Foster T. J.. 2001; Loss of clumping factor B fibrinogen binding activity by Staphylococcus aureus involves cessation of transcription, shedding and cleavage by metalloprotease. J Biol Chem276:29969–29978
    [Google Scholar]
  18. McDevitt D., Francois P., Vaudaux P., Foster T. J.. 1994; Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus . Mol Microbiol11:237–248
    [Google Scholar]
  19. McDevitt D., Francois P., Vaudaux P., Foster T. J.. 1995; Identification of the ligand-binding domain of the surface-located fibrinogen receptor (clumping factor) of Staphylococcus aureus . Mol Microbiol16:895–907
    [Google Scholar]
  20. McDevitt D., Nanavaty T., House-Pompeo K., Bell E., Turner N., McIntire L., Foster T., Hook M.. 1997; Characterization of the interaction between the Staphylococcus aureus clumping factor (ClfA) and fibrinogen. Eur J Biochem247:416–424
    [Google Scholar]
  21. McGavin M. H., Krajewska-Pietrasik D., Ryden C., Hook M.. 1993; Identification of a Staphylococcus aureus extracellular matrix-binding protein with broad specificity. Infect Immun61:2479–2485
    [Google Scholar]
  22. Medved L. V., Gorkun O. V., Privalov P. L.. 1983; Structural organization of C-terminal parts of fibrinogen A alpha-chains. FEBS Lett160:291–295
    [Google Scholar]
  23. Medved L. V., Gorkun O. V., Manyakov V. F., Belitser V. A.. 1985; The role of fibrinogen alpha C-domains in the fibrin assembly process. FEBS Lett181:109–112
    [Google Scholar]
  24. Miajlovic H., Loughman A., Brennan M., Cox D., Foster T. J.. 2007; Both complement- and fibrinogen-dependent mechanisms contribute to platelet aggregation mediated by Staphylococcus aureus clumping factor B. Infect Immun75:3335–3343
    [Google Scholar]
  25. Navarre W. W., Schneewind O.. 1994; Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in gram-positive bacteria. Mol Microbiol14:115–121
    [Google Scholar]
  26. Ni Eidhin D., Perkins S., Francois P., Vaudaux P., Hook M., Foster T. J.. 1998; Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus . Mol Microbiol30:245–257
    [Google Scholar]
  27. O'Brien L. M., Walsh E. J., Massey R. C., Peacock S. J., Foster T. J.. 2002; Staphylococcus aureus clumping factor B (ClfB) promotes adherence to human type I cytokeratin 10: implications for nasal colonization. Cell Microbiol4:759–770
    [Google Scholar]
  28. O'Connell D. P., Nanavaty T., McDevitt D., Gurusiddappa S., Hook M., Foster T. J.. 1998; The fibrinogen-binding MSCRAMM (clumping factor) of Staphylococcus aureus has a Ca2+-dependent inhibitory site. J Biol Chem273:6821–6829
    [Google Scholar]
  29. Palma M., Wade D., Flock M., Flock J. I.. 1998; Multiple binding sites in the interaction between an extracellular fibrinogen-binding protein from Staphylococcus aureus and fibrinogen. J Biol Chem273:13177–13181
    [Google Scholar]
  30. Peacock S. J., de Silva I., Lowy F. D.. 2001; What determines nasal carriage of Staphylococcus aureus ?. Trends Microbiol9:605–610
    [Google Scholar]
  31. Perkins S., Walsh E. J., Deivanayagam C. C., Narayana S. V., Foster T. J., Hook M.. 2001; Structural organization of the fibrinogen-binding region of the clumping factor B MSCRAMM of Staphylococcus aureus . J Biol Chem276:44721–44728
    [Google Scholar]
  32. Phonimdaeng P., O'Reilly M., Nowlan P., Bramley A. J., Foster T. J.. 1990; The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutants. Mol Microbiol4:393–404
    [Google Scholar]
  33. Ponnuraj K., Bowden M. G., Davis S., Gurusiddappa S., Moore D., Choe D., Xu Y., Hook M., Narayana S. V.. 2003; A “dock, lock, and latch” structural model for a staphylococcal adhesin binding to fibrinogen. Cell115:217–228
    [Google Scholar]
  34. Rudchenko S., Trakht I., Sobel J. H.. 1996; Comparative structural and functional features of the human fibrinogen alpha C domain and the isolated alpha C fragment. Characterization using monoclonal antibodies to defined COOH-terminal A alpha chain regions. J Biol Chem271:2523–2530
    [Google Scholar]
  35. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Labratory;
  36. Tsurupa G., Medved L.. 2001; Identification and characterization of novel tPA- and plasminogen-binding sites within fibrin(ogen) alpha C-domains. Biochemistry40:801–808
    [Google Scholar]
  37. Tsurupa G., Tsonev L., Medved L.. 2002; Structural organization of the fibrin(ogen) alpha C-domain. Biochemistry41:6449–6459
    [Google Scholar]
  38. Walsh E. J., O'Brien L. M., Liang X., Hook M., Foster T. J.. 2004; Clumping factor B, a fibrinogen-binding MSCRAMM (microbial surface components recognizing adhesive matrix molecules) adhesin of Staphylococcus aureus , also binds to the tail region of type I cytokeratin 10. J Biol Chem279:50691–50699
    [Google Scholar]
  39. Wann E. R., Gurusiddappa S., Hook M.. 2000; The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aureus is a bifunctional protein that also binds to fibrinogen. J Biol Chem275:13863–13871
    [Google Scholar]
  40. Weisel J. W., Medved L.. 2001; The structure and function of the α C domains of fibrinogen. Ann N Y Acad Sci936:312–327
    [Google Scholar]
  41. Yang Z., Kollman J. M., Pandi L., Doolittle R. F.. 2001; Crystal structure of native chicken fibrinogen at 2.7 Å resolution. Biochemistry40:12515–12523
    [Google Scholar]
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