1887

Abstract

Quorum sensing (QS) denotes a density-dependent mode of inter-bacterial communication based on signal transmitter molecules. Active QS is present during chronic infections with the opportunistic pathogen in immunocompromised patients. The authors have previously demonstrated a QS-regulated tolerance of biofilm bacteria to the antimicrobial properties of polymorphonuclear leukocytes (PMNs). The precise QS-regulated effect on the PMNs is, however, unknown. Incubation of human PMNs with supernatants from dense cultures showed that the QS-competent induced rapid necrosis of the PMNs. This mechanism was also observed in mouse lungs infected with , and in sputum obtained from -infected patients with cystic fibrosis. Evidence is presented that the necrotic effect was caused by rhamnolipids, production of which is QS controlled. The results demonstrate the potential of the QS system to facilitate infections with by disabling the PMNs, which are a major first line of defence of the host. Furthermore, the study emphasizes the inhibition of QS as a target for the treatment of infections with .

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2007-05-01
2019-10-19
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vol. , part 5, pp. 1329-1338

A time-lapse movie (MPEG format) showing the rapid death and disintegration of PMNs is avaialble here.



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