1887

Abstract

Transcription of the operon, which comprises genes involved in the late stage of galacturonate utilization, is known to be negatively regulated by the KdgR repressor. In this study, Northern analysis was carried out to demonstrate that the gene also negatively regulates the operon, encoding ketodeoxyuronate isomerase and ketodeoxygluconate reductase. It has also been demonstrated that expression of the operon can be induced by galacturonate and is subject to catabolite repression by glucose. The gene was found to be involved in this catabolite repression. Primer extension analysis identified a -like promoter sequence preceding . Gel mobility shift assays and DNase I footprinting analyses indicated that KdgR is capable of binding specifically to two sites within the intergenic region . Reporter gene analysis revealed that these two KdgR-binding sites function . One site is centred 33.5 bp upstream of the translational start site of and can serve as an operator for controlling expression of the operon. The other is centred 57.5 bp upstream of the translational start site of and can serve as an operator for controlling expression of the operon. Possible physiological significance of this regulation is discussed.

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2007-03-01
2019-10-24
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