1887

Abstract

Legionellae colonize biofilms in building water systems, yet little is known about their interaction with the organisms in these microbial communities. The role of type IV pili and the type II secretion pre-pilin peptidase was evaluated in a model biofilm system. strains 130b (wild-type), BS100 (a type IV pili mutant) and NU243 (a pre-pilin peptidase mutant) were assessed for attachment and retention in an established biofilm. Strains 130b and NU243 colonized the biofilm at a similar level while BS100 attached at a tenfold lower level. Over time, NU243 dropped below the level of detection while BS100 remained in the biofilm throughout the course of the experiment. The wild-type strain decreased but remained at a considerably higher level than either of the mutants. Inclusion of amoebae with BS100 allowed for attachment and retention at a level similar to 130b. NU243, which displays reduced intracellular replication, was able to establish itself and persist in the presence of amoebae. Thus, type IV pili and the pre-pilin peptidase facilitate colonization of biofilms but are not required in the presence of a host for intracellular replication.

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2006-12-01
2020-01-23
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References

  1. Abu Kwaik Y. 1998; Fatal attraction of mammalian cells to Legionella pneumophila . Mol Microbiol30:689–695[CrossRef]
    [Google Scholar]
  2. Aragon V, Kurtz S, Flieger A, Neumeister B, Cianciotto N. P. 2000; Secreted enzymatic activities of wild-type and pilD -deficient Legionella pneumophila . Infect Immun68:1855–1863[CrossRef]
    [Google Scholar]
  3. Bechet M, Blondeau R. 2003; Factors associated with the adherence and biofilm formation by Aeromonas caviae on glass surfaces. J Appl Microbiol94:1072–1078[CrossRef]
    [Google Scholar]
  4. Bowman E. K, Vass A. A, Mackowski R, Owen B. A, Tyndall R. L. 1996; Quantitation of free-living amoebae and bacterial populations in eyewash stations relative to flushing frequency. Am Ind Hyg Assoc J57:626–633[CrossRef]
    [Google Scholar]
  5. China B, Goffaux F. 1999; Secretion of virulence factors by Escherichia coli . Vet Res30:181–202
    [Google Scholar]
  6. Cianciotto N. P. 2001; Pathogenicity of Legionella pneumophila . Int J Med Microbiol291:331–343[CrossRef]
    [Google Scholar]
  7. Cowgill K. D, Lucas C. E, Benson R. F, Chamany S, Brown E. W, Fields B. S, Feikin D. R. 2005; Recurrence of Legionnaire's disease at a hotel in the United States Virgin Islands over a 20-year period. Clin Infect Dis40:1205–1207[CrossRef]
    [Google Scholar]
  8. Dalton H. M, March P. E. 1998; Molecular genetics of bacterial attachment and biofouling. Curr Opin Biotechnol9:252–255[CrossRef]
    [Google Scholar]
  9. DeLuca G, Stampi S, Lezzi L, Zanetti F. 1999; Effect of heat and acid decontamination treatments on the recovery of Legionella pneumophila from drinking water using two selective media. New Microbiol22:203–208
    [Google Scholar]
  10. Dorr J, Hurek T, Reinhold-Hurek B. 1998; Type IV pili are involved in plant-microbe and fungus-microbe interactions. Mol Microbiol30:7–17[CrossRef]
    [Google Scholar]
  11. Engleberg N. C, Drutz D. J, Eisenstein B. I. 1984; Cloning and expression of Legionella pneumophila antigens in Escherichia coli . Infect Immun44:222–227
    [Google Scholar]
  12. Entcheva-Dimitrov P, Spormann A. M. 2004; Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus . J Bacteriol186:8254–8266[CrossRef]
    [Google Scholar]
  13. Fields B. S. others 2002; The social life of legionellae. In Legionella pp. 135–142 Edited by Marre R.. Washington, DC: American Society for Microbiology;
  14. Fields B. S, Nerad T. A, Sawyer T. K, King C. H, Barbaree J. M, Martin W. T, Morrill W. E, Sanden G. N. 1990; Characterization of an axenic strain of Hartmannella vermiformis obtained from an investigation of nosocomial legionellosis. J Protozool37:581–583[CrossRef]
    [Google Scholar]
  15. Fields B. S, Benson R. F, Besser R. E. 2002; Legionella and Legionnaires' disease: 25 years of investigation. Clin Microbiol Rev15:506–526[CrossRef]
    [Google Scholar]
  16. Gao L. Y, Harb O. S, Abu Kwaik Y. 1997; Utilization of similar mechanisms by Legionella pneumophila to parasitize two evolutionarily distant host cells, mammalian macrophages and protozoa. Infect Immun65:4738–4746
    [Google Scholar]
  17. Gao L. Y, Stone B. J, Brieland J. K, Abu Kwaik Y. 1998; Different fates of Legionella pneumophila pmi and mil mutants within macrophages and alveolar epithelial cells. Microb Pathog25:291–306[CrossRef]
    [Google Scholar]
  18. Giltner C. L, Audette G. F, Kao D, Hodges R. S, Hassett D. J, Irvin R. T, van Schaik E. J. 2006; The Pseudomonas aeruginosa type IV pilin receptor binding domain functions as an adhesin for both biotic and abiotic surfaces. Mol Microbiol59:1083–1096[CrossRef]
    [Google Scholar]
  19. Kallstrom H, Islam M. S, Berggren P. O, Jonsson A. B. 1998; Cell signaling by the type IV pili of pathogenic Neisseria. J Biol Chem273:21777–21782[CrossRef]
    [Google Scholar]
  20. Le Thi T. T, Prigent-Combaret C, Dorel C, Lejeune P. 2001; First stages of biofilm formation: characterization and quantification of bacterial functions involved in colonization process. Methods Enzymol336:152–159
    [Google Scholar]
  21. Liles M. R, Edelstein P. H, Cianciotto N. P. 1999; The prepilin peptidase is required for protein secretion by and the virulence of the intracellular pathogen Legionella pneumophila . Mol Microbiol31:959–970[CrossRef]
    [Google Scholar]
  22. Moorthy S, Watnick P. I. 2004; Genetic evidence that the Vibrio cholerae monolayer is a distinct stage in biofilm development. Mol Microbiol52:573–587[CrossRef]
    [Google Scholar]
  23. Murga R, Forster T. S, Brown E, Pruckler J. M, Fields B. S, Donlan R. M. 2001; Role of biofilms in the survival of Legionella pneumophila in a model potable-water system. Microbiology147:3121–3126
    [Google Scholar]
  24. O'Toole G. A, Kolter R. 1998; Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol30:295–304[CrossRef]
    [Google Scholar]
  25. Romling U. 2001; Genetic and phenotypic analysis of multicellular behavior in Salmonella typhimurium . Methods Enzymol336:48–59
    [Google Scholar]
  26. Romling U, Sierralta W. D, Eriksson K, Normark S. 1998; Multicellular and aggregative behaviour of Salmonella typhimurium strains is controlled by mutations in the agfD promoter. Mol Microbiol28:249–264[CrossRef]
    [Google Scholar]
  27. Shapiro J. A. 1998; Thinking about bacterial populations as multicellular organisms. Annu Rev Microbiol52:81–104[CrossRef]
    [Google Scholar]
  28. Stanley N. R, Lazazzera B. A. 2004; Environmental signals and regulatory pathways that influence biofilm formation. Mol Microbiol52:917–924[CrossRef]
    [Google Scholar]
  29. Stone B. J, Abu Kwaik Y. 1998; Expression of multiple pili by Legionella pneumophila : identification and characterization of a type IV pilin gene and its role in adherence to mammalian and protozoan cells. Infect Immun66:1768–1775
    [Google Scholar]
  30. Stone B. J, Abu Kwaik Y. 1999; Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili. J Bacteriol181:1395–1402
    [Google Scholar]
  31. Strom M. S, Lory S. 1993; Structure-function and biogenesis of the type IV pili. Annu Rev Microbiol47:565–596[CrossRef]
    [Google Scholar]
  32. Wall D, Kaiser D. 1999; Type IV pili and cell motility. Mol Microbiol32:1–10[CrossRef]
    [Google Scholar]
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