1887

Abstract

Osmoregulated periplasmic glucans (OPGs) are general constituents of the envelope of Gram-negative bacteria. They are required for full virulence of bacterial phytopathogens such as , and . is a pectinolytic -proteobacterium that causes soft rot disease on a wide range of plant species. In addition to the loss of virulence, mutants exhibit a pleiotropic phenotype that affects motility, bile-salt resistance, exoenzyme secretion, exopolysaccharide synthesis and membrane lipid composition. This is believed to be the first proteomic analysis of an OPG-defective mutant of and it revealed that, in addition to the effects described, catabolic enzyme synthesis was enhanced and there was a greater abundance of some proteins catalysing the folding and degradation of proteins needed for various stress responses. Thus, in the mutant strain, loss of virulence was the result of a combination of envelope structure changes and cellular metabolism modifications.

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2007-03-01
2019-10-21
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