1887

Abstract

In this study, the regulatory mechanism of the (utilization of -ascorbic acid) operon, putatively responsible for transport and utilization of ascorbic acid in strain D39, is studied. β-Galactosidase assay data demonstrate that expression of the operon is increased in the presence of ascorbic acid as compared with the effects of other sugar sources including glucose. The operon consists of nine genes, including a transcriptional regulator UlaR, and is transcribed as a single transcriptional unit. We demonstrate the role of the transcriptional regulator UlaR as a transcriptional activator of the operon in the presence of ascorbic acid and show that activation of the operon genes by UlaR is CcpA-independent. Furthermore, we predict a 16 bp regulatory site (5′-AACAGTCCGCTGTGTA-3′) for UlaR in the promoter region of . Deletion of the half or full UlaR regulatory site in P confirmed that the UlaR regulatory site present in P is functional.

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2015-01-01
2020-09-18
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