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Volume 160, Issue 12

zinc-responsive proteins mediate bacterial defence against zinc overload, lysozyme and oxidative stress Free

Abstract

Two genes encoding the P-type ATPase EF1400 and the putative SapB protein EF0759 were previously shown to be strongly upregulated in the presence of high concentrations of zinc. In the present work, we showed that a Zn-responsive DNA-binding motif () is present in the promoter regions of these genes. Both proteins were further studied with respect to their involvement in zinc homeostasis and invasion of the host. EF0759 contributed to intramacrophage survival by an as-yet unknown mechanism(s). EF1400, here renamed ZntA, is an ATPase with specificity for zinc and plays a role in dealing with several host defences, i.e. zinc overload, oxidative stress and lysozyme; it provides cells with the ability to survive inside macrophages. As these three host defence mechanisms are important at several sites in the host, i.e. inside macrophages and in saliva, this work suggested that ZntA constitutes a crucial defence mechanism that is likely to contribute to the ability of this bacterium to endure life inside its host.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • Fundação para a Ciência e Tecnologia for Project (Award SFRH/BD/30362/2006, PEst-OE/EQB/LA0004/2011, PTDC/CVT/67270/2006 and POCTI/BIA-BCM/60643/2004)
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2014-12-01
2024-04-26
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Enterococcus faecalis zinc-responsive proteins mediate bacterial defence against zinc overload, lysozyme and oxidative stress
Microbiology 160, 2755 (2014); https://doi.org/10.1099/mic.0.080341-0
/content/journal/micro/10.1099/mic.0.080341-0
/content/journal/micro/10.1099/mic.0.080341-0
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