1887

Abstract

Filamentous PCC 7120 differentiates nitrogen-fixing specialized cells called heterocysts at regular intervals following removal of combined nitrogen from the medium. Phycobiliproteins are degraded during differentiation. Heterocyst differentiation was followed at the single cell level by using confocal fluorescence microscopy. The presence of an enhanced fluorescence emission peak from allophycocyanin (APC) indicates that the degradation of the phycobilisomes during nitrogen deprivation possibly initiates at the linker between APC and photosystem II in a bottom-to-top disassembly model. Furthermore, the fluorescence emission peak around 650 nm provides an advantageous marker to identify early candidates for differentiation.

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2013-02-01
2019-10-20
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References

  1. Allard J. F. , Hill A. L. , Rutenberg A. D. . ( 2007; ). Heterocyst patterns without patterning proteins in cyanobacterial filaments. . Dev Biol 312:, 427–434. [CrossRef] [PubMed]
    [Google Scholar]
  2. Buikema W. J. , Haselkorn R. . ( 1991; ). Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. . Genes Dev 5:, 321–330. [CrossRef] [PubMed]
    [Google Scholar]
  3. Buikema W. J. , Haselkorn R. . ( 2001; ). Expression of the Anabaena hetR gene from a copper-regulated promoter leads to heterocyst differentiation under repressing conditions. . Proc Natl Acad Sci U S A 98:, 2729–2734. [CrossRef] [PubMed]
    [Google Scholar]
  4. Callahan S. M. , Buikema W. J. . ( 2001; ). The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120. . Mol Microbiol 40:, 941–950. [CrossRef] [PubMed]
    [Google Scholar]
  5. Dines M. , Sendersky E. , David L. , Schwarz R. , Adir N. . ( 2008; ). Structural, functional, and mutational analysis of the NblA protein provides insight into possible modes of interaction with the phycobilisome. . J Bacteriol 283:, 30330–30340.
    [Google Scholar]
  6. Fay P. . ( 1992; ). Oxygen relations of nitrogen fixation in cyanobacteria. . Microbiol Rev 56:, 340–373.[PubMed]
    [Google Scholar]
  7. Flores E. , Herrero A. . ( 2010; ). Compartmentalized function through cell differentiation in filamentous cyanobacteria. . Nat Rev Microbiol 8:, 39–50. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gantt E. . ( 1981; ). Phycobilisomes. . Annu Rev Plant Physiol 32:, 327–347.[CrossRef]
    [Google Scholar]
  9. Glazer A. N. . ( 1985; ). Light harvesting by phycobilisomes. . Annu Rev Biophys Biophys Chem 14:, 47–77. [CrossRef] [PubMed]
    [Google Scholar]
  10. Golden J. W. , Yoon H. S. . ( 2003; ). Heterocyst development in Anabaena . . Curr Opin Microbiol 6:, 557–563. [CrossRef] [PubMed]
    [Google Scholar]
  11. Haselkorn R. . ( 1978; ). Heterocysts. . Annu Rev Plant Physiol 29:, 319–344. [CrossRef]
    [Google Scholar]
  12. Haselkorn R. . ( 2008; ). Cell–cell communication in filamentous cyanobacteria. . Mol Microbiol 70:, 783–785.[PubMed]
    [Google Scholar]
  13. Huang X. , Dong Y. , Zhao J. . ( 2004; ). HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS. . Proc Natl Acad Sci U S A 101:, 4848–4853. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kumar K. , Mella-Herrera R. A. , Golden J. W. . ( 2010; ). Cyanobacterial heterocysts. . Cold Spring Harb Perspect Biol 2:, a000315. [CrossRef] [PubMed]
    [Google Scholar]
  15. Liang J. , Scappino L. , Haselkorn R. . ( 1992; ). The patA gene product, which contains a region similar to CheY of Escherichia coli, controls heterocyst pattern formation in the cyanobacterium Anabaena 7120. . Proc Natl Acad Sci U S A 89:, 5655–5659. [CrossRef] [PubMed]
    [Google Scholar]
  16. Piven I. , Ajlani G. , Sokolenko A. . ( 2005; ). Phycobilisome linker proteins are phosphorylated in Synechocystis sp. PCC 6803. . J Biol Chem 280:, 21667–21672. [CrossRef] [PubMed]
    [Google Scholar]
  17. Risser D. D. , Callahan S. M. . ( 2009; ). Genetic and cytological evidence that heterocyst patterning is regulated by inhibitor gradients that promote activator decay. . Proc Natl Acad Sci U S A 106:, 19884–19888.[PubMed] [CrossRef]
    [Google Scholar]
  18. Toyoshima M. , Sasaki N. V. , Fujiwara M. , Ehira S. , Ohmori M. , Sato N. . ( 2010; ). Early candidacy for differentiation into heterocysts in the filamentous cyanobacterium Anabaena sp. PCC 7120. . Arch Microbiol 192:, 23–31. [CrossRef] [PubMed]
    [Google Scholar]
  19. Wolk C. P. . ( 1996; ). Heterocyst formation. . Annu Rev Genet 30:, 59–78. [CrossRef] [PubMed]
    [Google Scholar]
  20. Yoon H. S. , Golden J. W. . ( 1998; ). Heterocyst pattern formation controlled by a diffusible peptide. . Science 282:, 935–938. [CrossRef] [PubMed]
    [Google Scholar]
  21. Yoon H. S. , Golden J. W. . ( 2001; ). PatS and products of nitrogen fixation control heterocyst pattern. . J Bacteriol 183:, 2605–2613. [CrossRef] [PubMed]
    [Google Scholar]
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