1887

Abstract

Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial resistance to these toxicants were determined. Studies included wild-type cells as well as a strain exhibiting a modified monounsaturated fatty acid (MUFA) profile (accomplished by overexpressing the β-hydroxyacyl acyl carrier protein dehydratase-encoding gene, ). Levels of membrane MUFAs and oxidative damage markers decreased slightly upon toxicant exposure with a concomitant increase in cell resistance to these ROS-generating compounds. A direct relationship between MUFAs and lipid peroxidation was observed. The lower the MUFA the lower the peroxide levels, suggesting that MUFAs are targets for membrane lipid oxidation.

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2012-05-01
2022-01-29
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References

  1. Aoyagi K., Akiyama K., Tomida C., Gotoh M., Hirayama A., Takemura K., Ueda A., Nagase S., Koyama A., Narita M. ( 1999). Imaging of hydroperoxides in a rat glomerulus stimulated by puromycin aminonucleoside. Kidney IntSuppl 71 153–155 [View Article][PubMed]
    [Google Scholar]
  2. Bagchi D., Stohs S. J., Downs B. W., Bagchi M., Preuss H. G. ( 2002). Cytotoxicity and oxidative mechanisms of different forms of chromium. Toxicology 180:5–22 [View Article][PubMed]
    [Google Scholar]
  3. Bielski B. H., Arudi R. L., Sutherland M. W. ( 1983). A study of the reactivity of HO2/O2 with unsaturated fatty acids. J Biol Chem 258:4759–4761[PubMed]
    [Google Scholar]
  4. Birge C. H., Vagelos P. R. ( 1972). Acyl carrier protein. XVII. Purification and properties of -hydroxyacyl acyl carrier protein dehydrase. J Biol Chem 247:4930–4938[PubMed]
    [Google Scholar]
  5. Borchman D., Yappert M. C. ( 1998). Age-related lipid oxidation in human lenses. Invest Ophthalmol Vis Sci 39:1053–1058[PubMed]
    [Google Scholar]
  6. Bus J. S., Gibson J. E. ( 1984). Paraquat: model for oxidant-initiated toxicity. Environ Health Perspect 55:37–46 [View Article][PubMed]
    [Google Scholar]
  7. Cha M. K., Kim W. C., Lim C. J., Kim K., Kim I. H. ( 2004). Escherichia coli periplasmic thiol peroxidase acts as lipid hydroperoxide peroxidase and the principal antioxidative function during anaerobic growth. J Biol Chem 279:8769–8778 [View Article][PubMed]
    [Google Scholar]
  8. Clark D. P., DeMendoza D., Polacco M. L., Cronan J. E. Jr ( 1983). Beta-hydroxydecanoyl thio ester dehydrase does not catalyze a rate-limiting step in Escherichia coli unsaturated fatty acid synthesis. Biochemistry 22:5897–5902 [View Article][PubMed]
    [Google Scholar]
  9. Ercal N., Gurer-Orhan H., Aykin-Burns N. ( 2001). Toxic metals and oxidative stress part I: mechanisms involved in metal-induced oxidative damage. Curr Top Med Chem 1:529–539 [View Article][PubMed]
    [Google Scholar]
  10. Esterbauer H., Schaur R. J., Zollner H. ( 1991). Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 11:81–128 [View Article][PubMed]
    [Google Scholar]
  11. Imlay J. A. ( 2003). Pathways of oxidative damage. Annu Rev Microbiol 57:395–418 [View Article][PubMed]
    [Google Scholar]
  12. Johnson G. D. ( 1953). Correlation of color and constitution. I. 2, 4-dinitrophenylhydrazones. J Am Chem Soc 75:2720–2723 [View Article]
    [Google Scholar]
  13. Loidl-Stahlhofen A., Kern W., Spiteller G. ( 1995). Gas chromatographic-electron impact mass spectrometric screening procedure for unknown hydroxyaldehydic lipid peroxidation products after pentafluorobenzyloxime derivatization. J Chromatogr B Biomed Appl 673:1–14 [View Article][PubMed]
    [Google Scholar]
  14. Macomber L., Hausinger R. P. ( 2011). Mechanisms of nickel toxicity in microorganisms. Metallomics 3:1153–1162 [View Article][PubMed]
    [Google Scholar]
  15. Maness P. C., Smolinski S., Blake D. M., Huang Z., Wolfrum E. J., Jacoby W. A. ( 1999). Bactericidal activity of photocatalytic TiO2 reaction: toward an understanding of its killing mechanism. Appl Environ Microbiol 65:4094–4098[PubMed]
    [Google Scholar]
  16. Masaki N., Kyle M. E., Farber J. L. ( 1989). tert-Butyl hydroperoxide kills cultured hepatocytes by peroxidizing membrane lipids. Arch Biochem Biophys 269:390–399 [View Article][PubMed]
    [Google Scholar]
  17. Messner K. R., Imlay J. A. ( 1999). The identification of primary sites of superoxide and hydrogen peroxide formation in the aerobic respiratory chain and sulfite reductase complex of Escherichia coli . J Biol Chem 274:10119–10128 [View Article][PubMed]
    [Google Scholar]
  18. Pérez J. M., Calderón I. L., Arenas F. A., Fuentes D. E., Pradenas G. A., Fuentes E. L., Sandoval J. M., Castro M. E., Elías A. O., Vásquez C. C. ( 2007). Bacterial toxicity of potassium tellurite: unveiling an ancient enigma. PLoS ONE 2:e211 [View Article][PubMed]
    [Google Scholar]
  19. Pérez J. M., Arenas F. A., Pradenas G. A., Sandoval J. M., Vásquez C. C. ( 2008). Escherichia coli YqhD exhibits aldehyde reductase activity and protects from the harmful effect of lipid peroxidation-derived aldehydes. J Biol Chem 283:7346–7353 [View Article][PubMed]
    [Google Scholar]
  20. Rontani J. F. ( 1998). Photodegradation of unsaturated fatty acids in senescent cells of phytoplankton: photoproduct structural identification and mechanistic aspects. J Photochem Photobiol Chem 114:37–44 [View Article]
    [Google Scholar]
  21. Royall J. A., Ischiropoulos H. ( 1993). Evaluation of 2′,7′-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular H2O2 in cultured endothelial cells. Arch Biochem Biophys 302:348–355 [View Article][PubMed]
    [Google Scholar]
  22. Semchyshyn H., Bagnyukova T., Storey K., Lushchak V. ( 2005). Hydrogen peroxide increases the activities of soxRS regulon enzymes and the levels of oxidized proteins and lipids in Escherichia coli . Cell Biol Int 29:898–902 [View Article][PubMed]
    [Google Scholar]
  23. Stadtman E. R., Levine R. L. ( 2000). Protein oxidation. Ann N Y Acad Sci 899:191–208 [View Article][PubMed]
    [Google Scholar]
  24. Vogel H. J., Bonner D. M. ( 1956). Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem 218:97–106[PubMed]
    [Google Scholar]
  25. Yan L. J. ( 2009). Analysis of oxidative modification of proteins. Curr Protoc Protein Sci Chapter 14:t14–, 4[PubMed]
    [Google Scholar]
  26. Yoon S. J., Park J. E., Yang J. H., Park J. W. ( 2002). OxyR regulon controls lipid peroxidation-mediated oxidative stress in Escherichia coli . J Biochem Mol Biol 35:297–301 [View Article][PubMed]
    [Google Scholar]
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