1887

Abstract

Polynucleotide phosphorylase (PNPase), a multifunctional protein, is a 3′→5′ exoribonuclease or exoDNase in the presence of inorganic phosphate (P), and extends a 3′-OH of RNA or ssDNA in the presence of ADP or dADP. In , PNPase is known to protect against HO- and mitomycin C-induced damage. Recent reports show that PNPase is required for repair of HO-induced double-strand breaks. Here we show that absence of PNPase makes cells sensitive to UV, indicating that PNPase has a role in survival of UV radiation damage. Analyses of various DNA repair pathways show that in the absence of nucleotide excision repair, survival of UV radiation depends critically on PNPase function. Consequently, , and strains show hypersensitivity to UV radiation. Whereas the mutation is non-epistatic to , and mutations with respect to the UV-sensitivity phenotype, it is epistatic to , and mutations, implicating it in the recombinational repair process.

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2012-05-01
2019-08-21
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