1887

Abstract

which causes serious infections in immunocompromised patients, produces numerous virulence factors, including exotoxin A and the siderophore pyoverdine. As production of these virulence factors is influenced by the host environment, we examined the effect serum has on global transcription within strain PAO1 at different phases of growth in an iron-deficient medium. At early exponential phase, serum significantly enhanced expression of 138 genes, most of which are repressed by iron, including , and the pyoverdine synthesis genes. However, serum did not interfere with the repression of these genes by iron. Serum enhanced expression in a mutant of PAO1 but not in a mutant. The serum iron-binding protein apotransferrin, but not ferritin, enhanced and expression. However, in PAO1 grown in a chemically defined medium that contains no iron, serum but not apotransferrin enhanced and expression. While complement inactivation failed to eliminate this effect, albumin absorption reduced the effect of serum on and expression in the iron-deficient medium chelexed tryptic soy broth dialysate. Additionally, albumin absorption eliminated the effect of serum on and expression in the chemically defined medium. These results suggest that serum enhances the expression of iron-controlled genes by two mechanisms: one through apotransferrin and another one through albumin.

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2012-02-01
2020-05-26
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