1887

Abstract

Most studies on fungal biofilms have focused on in yeasts and in mycelial fungi. To the authors’ knowledge, biofilm formation by zygomycetes has not been reported previously. In this study, the biofilm-forming capacity of , , and was evaluated At appropriate seeding spore densities, (10 c.f.u. ml), (10 c.f.u. ml) and (10 c.f.u. ml) produced highly intertwined, adherent structures on flat-bottomed polystyrene microtitre plates after 24 h at 37 °C. The adhered fungal hyphae were encased in an extracellular matrix, as confirmed by phase-contrast and confocal microscopy. The thickness of , and biofilms was 109.67±10.02, 242±23.07 and 197±9.0 µm (mean±), respectively. Biochemical characterization of the biofilm matrix indicated the presence of glucosamine, constituting 74.54–82.22 % of its dry weight, acetylglucosamine, glucose and proteins. Adherence and biofilm formation were not observed in . Although spores germinated at all three seeding densities tested (1×10, 1×10 and 1×10 c.f.u. ml), no significant difference was observed (>0.05) between the of wells inoculated with and the cut-off for biofilm detection. This study highlights the potential for biofilm formation by at least three medically important species of zygomycetes.

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2011-09-01
2020-07-08
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