1887

Abstract

serovar Typhimurium has at least nine multidrug efflux pumps. Among these, AcrAB is constitutively expressed and is the most efficient, playing a role in both drug resistance and virulence. The locus is induced by indole, -conditioned medium, and bile salts. This induction is dependent on RamA through the binding sequence in the upstream region of that binds RamA. In the present study, we made a detailed investigation of the and induction mechanisms in in response to indole, a biological oxidant for bacteria. We found that and induction in response to indole is dependent on RamR. However, the cysteine residues of RamR do not play a role in the induction of in response to indole, and the oxidative effect of indole is therefore not related to induction via RamR. Furthermore, we showed that paraquat, a superoxide generator, induces but not . We further discovered that the mechanism of induction in response to paraquat is dependent on SoxS. The data indicate that there are at least two independent induction pathways for in response to extracellular signals such as indole and paraquat. We propose that utilizes these regulators for induction in response to extracellular signals in order to adapt itself to environmental conditions.

Keyword(s): R6G, rhodamine 6G
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2011-03-01
2019-10-20
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